論文 - 詳細
| RRC ID | 1520 |
|---|---|
| 著者 | Yano F, Kugimiya F, Ohba S, Ikeda T, Chikuda H, Ogasawara T, Ogata N, Takato T, Nakamura K, Kawaguchi H, Chung UI. |
| タイトル | The canonical Wnt signaling pathway promotes chondrocyte differentiation in a Sox9-dependent manner. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
To better understand the role of the canonical Wnt signaling pathway in cartilage development, we adenovirally expressed a constitutively active (ca) or a dominant negative (dn) form of lymphoid enhancer factor-1 (LEF-1), the main nuclear effector of the pathway, in undifferentiated mesenchymal cells, chondrogenic cells, and primary chondrocytes, and examined the expression of markers for chondrogenic differentiation and hypertrophy. caLEF-1 and LiCl, an activator of the canonical pathway, promoted both chondrogenic differentiation and hypertrophy, whereas dnLEF-1 and the gene silencing of beta-catenin suppressed LiCl-promoted effects. To investigate whether these effects were dependent on Sox9, a master regulator of cartilage development, we stimulated Sox9-deficient ES cells with the pathway. caLEF-1 and LiCl promoted both chondrogenic differentiation and hypertrophy in wild-type, but not in Sox9-deficient, cells. The response of Sox9-deficient cells was restored by the adenoviral expression of Sox9. Thus, the canonical Wnt signaling pathway promotes chondrocyte differentiation in a Sox9-dependent manner. |
| 巻・号 | 333(4) |
| ページ | 1300-8 |
| 公開日 | 2005-8-12 |
| DOI | 10.1016/j.bbrc.2005.06.041 |
| PII | S0006-291X(05)01256-8 |
| PMID | 15979579 |
| MeSH | Animals Cell Differentiation / physiology Chondrocytes / cytology* Chondrocytes / metabolism* Chondrogenesis / physiology* High Mobility Group Proteins / metabolism* Intercellular Signaling Peptides and Proteins / metabolism* Mesenchymal Stem Cells / cytology* Mesenchymal Stem Cells / metabolism* Mice Mice, Inbred C57BL SOX9 Transcription Factor Signal Transduction / physiology Transcription Factors / metabolism* Wnt Proteins |
| IF | 2.985 |
| 引用数 | 111 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 10T1/2(RCB0247) ATDC5(RCB0565) |