Reference - Detail
| RRC ID | 1523 |
|---|---|
| Author | Ishizuka M, Kawate H, Takayanagi R, Ohshima H, Tao RH, Hagiwara H. |
| Title | A zinc finger protein TZF is a novel corepressor of androgen receptor. |
| Journal | Biochem Biophys Res Commun |
| Abstract |
Steroid hormones control the transcriptional activity of target genes mediated by intracellular nuclear receptors, and these transcriptional activities are modulated by the combination with coactivators and corepressors. We found in this study that testicular zinc finger protein (TZF) that was a nuclear protein with a zinc finger motif of the Cys2-His2 type was a novel corepressor of androgen receptor (AR). Fusion protein with green fluorescence protein GFP formed the specific foci in nuclei and TZF-dependent foci were located close to the splicing factor compartment. In addition, TZF was recruited into AR subnuclear foci after the treatment of dihydrotestosterone. Furthermore, we revealed that TZF bound to the activation function-1 (AF-1) domain (N-terminal transactivating domain) of AR protein. Transient over-expression of TZF in COS-7 cells or LNCaP human prostatic cancer cell resulted in decreased AR activity in a ligand-dependent fashion. Moreover, a transcriptional corepressor N-CoR additively decreased the transcriptional activity of AR with TZF. These findings suggest that TZF might be a novel corepressor of AR. |
| Volume | 331(4) |
| Pages | 1025-31 |
| Published | 2005-6-17 |
| DOI | 10.1016/j.bbrc.2005.04.024 |
| PII | S0006-291X(05)00783-7 |
| PMID | 15882980 |
| MeSH | Androgen Receptor Antagonists* Animals COS Cells Protein Binding RNA Splicing Receptors, Androgen / metabolism Receptors, Androgen / physiology Repressor Proteins / metabolism Repressor Proteins / physiology* Transcriptional Activation / physiology Zinc Fingers* |
| IF | 2.985 |
| Times Cited | 13 |
| WOS Category | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | Wikipedia |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | COS-7(RCB0539) |