RRC ID 15311
Author Takanashi K, Sugiyama A, Sato S, Tabata S, Yazaki K.
Title LjABCB1, an ATP-binding cassette protein specifically induced in uninfected cells of Lotus japonicus nodules.
Journal J. Plant Physiol.
Abstract Legume plants develop root nodules through symbiosis with rhizobia, and fix atmospheric nitrogen in this symbiotic organ. Development of root nodules is regulated by many metabolites including phytohormones. Previously, we reported that auxin is strongly involved in the development of the nodule vascular bundle and lenticel formation on the nodules of Lotus japonicus. Here we show that an ATP-binding cassette (ABC) protein, LjABCB1, which is a homologue of Arabidopsis auxin transporter AtABCB4, is specifically expressed during nodulation of L. japonicus. A reporter gene analysis indicated that the expression of LjABCB1 was restricted to uninfected cells adjacent to infected cells in the nodule, while no expression was observed in shoot apical meristems or root tips, in which most auxin transporter genes are expressed. The auxin transport activity of LjABCB1 was confirmed using a heterologous expression system.
Volume 169(3)
Pages 322-6
Published 2012-2-15
DOI 10.1016/j.jplph.2011.11.008
PII S0176-1617(11)00463-9
PMID 22209217
MeSH ATP-Binding Cassette, Sub-Family B, Member 1 / biosynthesis* ATP-Binding Cassette, Sub-Family B, Member 1 / genetics Amino Acid Sequence Base Sequence Carrier Proteins Gene Expression Regulation, Plant Genes, Plant Loteae / genetics Loteae / growth & development Loteae / metabolism* Nitrogen Fixation / genetics* Plant Proteins / biosynthesis* Plant Proteins / genetics Rhizobium / metabolism Root Nodules, Plant / growth & development Root Nodules, Plant / metabolism* Symbiosis
IF 2.833
Times Cited 9
Lotus / Glycine Miyakojima MG-20