RRC ID 15315
Author Bahaji A, Ovecka M, Bárány I, Risueño MC, Muñoz FJ, Baroja-Fernández E, Montero M, Li J, Hidalgo M, Sesma MT, Ezquer I, Testillano PS, Pozueta-Romero J.
Title Dual targeting to mitochondria and plastids of AtBT1 and ZmBT1, two members of the mitochondrial carrier family.
Journal Plant Cell Physiol
Abstract Zea mays and Arabidopsis thaliana Brittle 1 (ZmBT1 and AtBT1, respectively) are members of the mitochondrial carrier family. Although they are presumed to be exclusively localized in the envelope membranes of plastids, confocal fluorescence microscopy analyses of potato, Arabidopsis and maize plants stably expressing green fluorescent protein (GFP) fusions of ZmBT1 and AtBT1 revealed that the two proteins have dual localization to plastids and mitochondria. The patterns of GFP fluorescence distribution observed in plants stably expressing GFP fusions of ZmBT1 and AtBT1 N-terminal extensions were fully congruent with that of plants expressing a plastidial marker fused to GFP. Furthermore, the patterns of GFP fluorescence distribution and motility observed in plants expressing the mature proteins fused to GFP were identical to those observed in plants expressing a mitochondrial marker fused to GFP. Electron microscopic immunocytochemical analyses of maize endosperms using anti-ZmBT1 antibodies further confirmed that ZmBT1 occurs in both plastids and mitochondria. The overall data showed that (i) ZmBT1 and AtBT1 are dually targeted to mitochondria and plastids; (ii) AtBT1 and ZmBT1 N-terminal extensions comprise targeting sequences exclusively recognized by the plastidial compartment; and (iii) targeting sequences to mitochondria are localized within the mature part of the BT1 proteins.
Volume 52(4)
Pages 597-609
Published 2011-4-1
DOI 10.1093/pcp/pcr019
PII pcr019
PMID 21330298
MeSH Animals Arabidopsis / genetics Arabidopsis / metabolism* Arabidopsis / ultrastructure Biological Transport Endosperm / metabolism Endosperm / ultrastructure Gene Expression Regulation, Plant Genetic Markers Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Microscopy, Confocal Microscopy, Immunoelectron Mitochondria / metabolism Mitochondria / ultrastructure Mitochondrial Membrane Transport Proteins / genetics Mitochondrial Membrane Transport Proteins / immunology Mitochondrial Membrane Transport Proteins / metabolism* Nucleotide Transport Proteins / genetics Nucleotide Transport Proteins / immunology Nucleotide Transport Proteins / metabolism* Plant Proteins / genetics Plant Proteins / immunology Plant Proteins / metabolism Plants, Genetically Modified / enzymology Plants, Genetically Modified / genetics Plants, Genetically Modified / metabolism Plastids / metabolism* Plastids / ultrastructure Rabbits Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Solanum tuberosum / genetics Solanum tuberosum / metabolism* Solanum tuberosum / ultrastructure Zea mays / genetics Zea mays / metabolism* Zea mays / ultrastructure
IF 4.062
Times Cited 25
Arabidopsis / Cultured plant cells, genes pda01711