RRC ID 15317
著者 Voegele A, Linkies A, Müller K, Leubner-Metzger G.
タイトル Members of the gibberellin receptor gene family GID1 (GIBBERELLIN INSENSITIVE DWARF1) play distinct roles during Lepidium sativum and Arabidopsis thaliana seed germination.
ジャーナル J Exp Bot
Abstract Germination of endospermic seeds is partly regulated by the micropylar endosperm, which acts as constraint to radicle protrusion. Gibberellin (GA) signalling pathways control coat-dormancy release, endosperm weakening, and organ expansion during seed germination. Three GIBBERELLIN INSENSITIVE DWARF1 (GID1) GA receptors are known in Arabidopsis thaliana: GID1a, GID1b, and GID1c. Molecular phylogenetic analysis of angiosperm GID1s reveals that they cluster into two eudicot (GID1ac, GID1b) groups and one monocot group. Eudicots have at least one gene from each of the two groups, indicating that the different GID1 receptors fulfil distinct roles during plant development. A comparative Brassicaceae approach was used, in which gid1 mutant and whole-seed transcript analyses in Arabidopsis were combined with seed-tissue-specific analyses of its close relative Lepidium sativum (garden cress), for which three GID1 orthologues were cloned. GA signalling via the GID1ac receptors is required for Arabidopsis seed germination, GID1b cannot compensate for the impaired germination of the gid1agid1c mutant. Transcript expression patterns differed temporarily, spatially, and hormonally, with GID1b being distinct from GID1ac in both species. Endosperm weakening is mediated, at least in part, through GA-induced genes encoding cell-wall-modifying proteins. A suppression subtraction hybridization (SSH) cDNA library enriched for sequences that are highly expressed during early germination in the micropylar endosperm contained expansins and xyloglucan endo-transglycosylases/hydrolases (XTHs). Their transcript expression patterns in both species strongly suggest that they are regulated by distinct GID1-mediated GA signalling pathways. The GID1ac and GID1b pathways seem to fulfil distinct regulatory roles during Brassicaceae seed germination and seem to control their downstream targets distinctly.
巻・号 62(14)
ページ 5131-47
公開日 2011-10-1
DOI 10.1093/jxb/err214
PII err214
PMID 21778177
PMC PMC3193015
MeSH Arabidopsis / classification Arabidopsis / genetics Arabidopsis / growth & development Arabidopsis / metabolism* Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Germination* Gibberellins / metabolism Lepidium sativum / classification Lepidium sativum / genetics Lepidium sativum / growth & development Lepidium sativum / metabolism* Molecular Sequence Data Multigene Family Phylogeny Plant Growth Regulators / metabolism Receptors, Cell Surface / genetics Receptors, Cell Surface / metabolism* Seeds / genetics Seeds / growth & development* Seeds / metabolism
IF 5.908
引用数 70
WOS 分野 PLANT SCIENCES
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 psi00014 psi00015 psi00016 psi00017 psi00018 psi00019 psi00020 psi00021