| RRC ID |
15322
|
| Author |
Van Hove J, Fouquaert E, Smith DF, Proost P, Van Damme EJ.
|
| Title |
Lectin activity of the nucleocytoplasmic EUL protein from Arabidopsis thaliana.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
The Euonymus lectin (EUL) domain was recognized as the structural motif for a novel class of putative carbohydrate binding proteins. Confocal microscopy demonstrated that the lectin from Euonymus europaeus (EEA) as well as the EUL protein from Arabidopsis thaliana (ArathEULS3) are located in the nucleocytoplasmic compartment of the plant cell. ArathEULS3 as well as its EUL domain were successfully expressed in Pichia pastoris and purified. The EUL domain from Arabidopsis interacts with glycan structures containing Lewis Y, Lewis X and lactosamine, indicating that it can be considered a true lectin domain. Despite the high sequence identity between the EUL domains in EEA and ArathEULS3, both domains recognize different carbohydrate structures.
|
| Volume |
414(1)
|
| Pages |
101-5
|
| Published |
2011-10-14
|
| DOI |
10.1016/j.bbrc.2011.09.031
|
| PII |
S0006-291X(11)01620-2
|
| PMID |
21945438
|
| PMC |
PMC3195871
|
| MeSH |
Amino Acid Sequence
Amino Sugars / chemistry
Amino Sugars / metabolism
Antimicrobial Cationic Peptides / chemistry
Antimicrobial Cationic Peptides / genetics
Antimicrobial Cationic Peptides / metabolism*
Arabidopsis / chemistry
Arabidopsis / genetics
Arabidopsis / metabolism*
Arabidopsis Proteins / chemistry
Arabidopsis Proteins / genetics
Arabidopsis Proteins / metabolism*
Cell Nucleus / metabolism
Cloning, Molecular
Cytoplasm / metabolism
Lectins / chemistry
Lectins / genetics
Lectins / metabolism*
Lewis Blood Group Antigens / chemistry
Lewis Blood Group Antigens / metabolism
Lewis X Antigen / chemistry
Lewis X Antigen / metabolism
Molecular Sequence Data
Pichia
Plant Lectins / chemistry
Plant Lectins / genetics
Plant Lectins / metabolism*
Protein Structure, Tertiary
|
| IF |
2.985
|
| Times Cited |
20
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Arabidopsis / Cultured plant cells, genes |
pda02666 |
