RRC ID |
15345
|
著者 |
Wang Y, Zhang P, Fujii H, Banno Y, Yamamoto K, Aso Y.
|
タイトル |
Proteomic studies of lipopolysaccharide-induced polypeptides in the silkworm, Bombyx mori.
|
ジャーナル |
Biosci Biotechnol Biochem
|
Abstract |
Silkworm larvae at the 5th instar were injected with lipopolysaccharide from Escherichia coli and inducible polypeptides were examined within a pI range of 3-10 and a size range of 14-97 kDa by proteomics, including peptide mass fingerprinting. No polypeptides were induced in the midgut. FB1 and H1-4 polypeptides were significantly induced in fat body and hemolymph, respectively. FB1 and H1 were estimated to be antitrypsin and serpin-2 proteinase inhibitors respectively. H2 and H3 were novel polypeptides. H4 was estimated to be attacin antibacterial polypeptide with high coverage of sequence. The amounts of all the induced polypeptides decreased at 48 h after the injection.
|
巻・号 |
68(8)
|
ページ |
1821-3
|
公開日 |
2004-8-1
|
DOI |
10.1271/bbb.68.1821
|
PII |
JST.JSTAGE/bbb/68.1821
|
PMID |
15322375
|
MeSH |
Animals
Bombyx / metabolism*
Electrophoresis, Gel, Two-Dimensional
Escherichia coli
Fat Body / metabolism*
Hemolymph / metabolism*
Larva / metabolism
Lipopolysaccharides / pharmacology*
Peptides / metabolism
Proteome / drug effects*
Proteome / metabolism
|
IF |
1.516
|
引用数 |
19
|
WOS 分野
|
CHEMISTRY, APPLIED
FOOD SCIENCE & TECHNOLOGY
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
カイコ |
p50 |