| RRC ID |
1535
|
| Author |
Pang Z, Otaka K, Maoka T, Hidaka K, Ishijima S, Oda M, Ohnishi M.
|
| Title |
Structure of beta-glucan oligomer from laminarin and its effect on human monocytes to inhibit the proliferation of U937 cells.
|
| Journal |
Biosci Biotechnol Biochem
|
| Abstract |
We analyzed the human monocyte-stimulating ability of laminarin from Eisenia bicyclis, lichenan from Cetraria islandica, and their oligomers depolymerized with endo-1,3-beta-glucanase from Arthrobacter sp. The respective beta-glucan oligomers with different degrees of polymerization (DP) were fractionated from hydrolytic products of laminarin and lichenan using gel-filtration chromatography. The monocyte-conditioned medium pre-cultured in the presence of a fraction of beta-glucan oligomer (DP>/=8) from laminarin exhibited inhibitory activity against the proliferation of human myeloid leukemia U937 cells, while those pre-cultured with other beta-glucan oligomers and the original laminarin and lichenan showed little or no activity. NMR analysis indicated that the beta-glucan oligomer (DP>/=8) has an average DP value of 13, and its ratio of beta-1,3- to beta-1,6-linkages in glucopyranose units was estimated to be 1.3:1. These results indicate that the beta-1,3-glucan oligomer with a higher content of beta-1,6-linkage stimulates monocytes to inhibit the proliferation of U937 cells.
|
| Volume |
69(3)
|
| Pages |
553-8
|
| Published |
2005-3-1
|
| DOI |
10.1271/bbb.69.553
|
| PII |
JST.JSTAGE/bbb/69.553
|
| PMID |
15784984
|
| MeSH |
Biopolymers / chemistry*
Biopolymers / pharmacology
Cell Division / drug effects*
Glucans
Humans
Molecular Structure
Monocytes / cytology
Monocytes / drug effects*
Nuclear Magnetic Resonance, Biomolecular
Polysaccharides / chemistry*
U937 Cells
beta-Glucans / chemistry*
beta-Glucans / pharmacology
|
| IF |
1.516
|
| Times Cited |
64
|
|
WOS Category
|
CHEMISTRY, APPLIED
FOOD SCIENCE & TECHNOLOGY
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
U-937 DE-4(RCB0435) |
