RRC ID 1536
Author Suemori H, Yasuchika K, Hasegawa K, Fujioka T, Tsuneyoshi N, Nakatsuji N.
Title Efficient establishment of human embryonic stem cell lines and long-term maintenance with stable karyotype by enzymatic bulk passage.
Journal Biochem. Biophys. Res. Commun.
Abstract Human ES (hES) cell lines are considered to be a valuable resource for medical research and for applications in cell therapy and drug discovery. For such utilization of hES cells to be realized, however, protocols involved in the use of hES cells, such as those for establishment, propagation, and cryopreservation, have still to be improved. Here, we report on an efficient method for the establishment of hES cell lines and its detailed characterization. Additionally, we developed a new bulk-passaging technique that preserves the karyotypic integrity of hES cell lines when maintained in culture for up to 2 years. Finally, we show that a simplified vitrification cryopreservation technique is vastly superior to standard slow-cooling methods with respect to cell viability. These results provide valuable information that will assist in achieving the goal of the large-scale hES cell culture required for the application of hES cells to disease therapy.
Volume 345(3)
Pages 926-32
Published 2006-7-7
DOI 10.1016/j.bbrc.2006.04.135
PII S0006-291X(06)00936-3
PMID 16707099
MeSH Animals Cell Culture Techniques / methods* Cell Differentiation Cell Line Embryo, Mammalian / cytology* Female Humans Karyotyping / methods* Male Mice Mice, SCID Stem Cells / cytology* Teratoma / metabolism
IF 2.705
Times Cited 208
Human embryonic stem cells KhES-1 KhES-2 KhES-3