論文 - 詳細
| RRC ID | 15381 |
|---|---|
| 著者 | Fan SJ, Marchand V, Ephrussi A. |
| タイトル | Drosophila Ge-1 promotes P body formation and oskar mRNA localization. |
| ジャーナル | PLoS One |
| Abstract |
mRNA localization coupled with translational control is a widespread and conserved strategy that allows the localized production of proteins within eukaryotic cells. In Drosophila, oskar (osk) mRNA localization and translation at the posterior pole of the oocyte are essential for proper patterning of the embryo. Several P body components are involved in osk mRNA localization and translational repression, suggesting a link between P bodies and osk RNPs. In cultured mammalian cells, Ge-1 protein is required for P body formation. Combining genetic, biochemical and immunohistochemical approaches, we show that, in vivo, Drosophila Ge-1 (dGe-1) is an essential gene encoding a P body component that promotes formation of these structures in the germline. dGe-1 partially colocalizes with osk mRNA and is required for osk RNP integrity. Our analysis reveals that although under normal conditions dGe-1 function is not essential for osk mRNA localization, it becomes critical when other components of the localization machinery, such as staufen, Drosophila decapping protein 1 and barentsz are limiting. Our findings suggest an important role of dGe-1 in optimization of the osk mRNA localization process required for patterning the Drosophila embryo. |
| 巻・号 | 6(5) |
| ページ | e20612 |
| 公開日 | 2011-5-31 |
| DOI | 10.1371/journal.pone.0020612 |
| PII | PONE-D-11-02925 |
| PMID | 21655181 |
| PMC | PMC3105097 |
| MeSH | Animals Animals, Genetically Modified Blotting, Western Caspases Drosophila / genetics Drosophila / metabolism* Drosophila Proteins / genetics Drosophila Proteins / metabolism* Immunoprecipitation In Situ Hybridization, Fluorescence RNA, Messenger / metabolism* RNA-Binding Proteins / genetics RNA-Binding Proteins / metabolism Reverse Transcriptase Polymerase Chain Reaction |
| IF | 2.74 |
| 引用数 | 15 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | P{w[+mC]=GSV2}GS5005 (DGRC#200633) |