Reference - Detail
| RRC ID | 15411 |
|---|---|
| Author | Nishimoto M, Kitaoka M. |
| Title | Identification of N-acetylhexosamine 1-kinase in the complete lacto-N-biose I/galacto-N-biose metabolic pathway in Bifidobacterium longum. |
| Journal | Appl Environ Microbiol |
| Abstract |
We have determined the functions of the enzymes encoded by the lnpB, lnpC, and lnpD genes, located downstream of the lacto-N-biose phosphorylase gene (lnpA), in Bifidobacterium longum JCM1217. The lnpB gene encodes a novel kinase, N-acetylhexosamine 1-kinase, which produces N-acetylhexosamine 1-phosphate; the lnpC gene encodes UDP-glucose hexose 1-phosphate uridylyltransferase, which is also active on N-acetylhexosamine 1-phosphate; and the lnpD gene encodes a UDP-glucose 4-epimerase, which is active on both UDP-galactose and UDP-N-acetylgalactosamine. These results suggest that the gene operon lnpABCD encodes a previously undescribed lacto-N-biose I/galacto-N-biose metabolic pathway that is involved in the intestinal colonization of bifidobacteria and that utilizes lacto-N-biose I from human milk oligosaccharides or galacto-N-biose from mucin sugars. |
| Volume | 73(20) |
| Pages | 6444-9 |
| Published | 2007-10-1 |
| DOI | 10.1128/AEM.01425-07 |
| PII | AEM.01425-07 |
| PMID | 17720833 |
| PMC | PMC2075035 |
| MeSH | Acetylglucosamine / analogs & derivatives* Acetylglucosamine / metabolism Bacterial Proteins / genetics Bacterial Proteins / metabolism Bifidobacterium / enzymology* Bifidobacterium / genetics Disaccharides / metabolism* Escherichia coli / enzymology Escherichia coli / genetics Hexosamines / metabolism* Molecular Sequence Data Operon Phosphotransferases / metabolism* Sequence Analysis, DNA |
| IF | 4.016 |
| Times Cited | 125 |
| WOS Category | BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY |
| Resource | |
| General Microbes | JCM 1217 |