| RRC ID |
15413
|
| 著者 |
Taki H, Syutsubo K, Mattison RG, Harayama S.
|
| タイトル |
Identification and characterization of o-xylene-degrading Rhodococcus spp. which were dominant species in the remediation of o-xylene-contaminated soils.
|
| ジャーナル |
Biodegradation
|
| Abstract |
Soils contaminated with o-xylene were more difficult to bioremediate than those contaminated with other BTEX hydrocarbons (benzene, toluene, ethylbenzene, m-xylene and p-xylene). In order to identify microorganisms responsible for o-xylene degradation in soil, microbial community structure analyses were carried out with two soil samples in the presence of o-xylene and mineral nutrients. In two different soil samples, Rhodococcus opacus became abundant. We were also able to isolate o-xylene degrading Rhodococcus species from these soil samples. A primer set was developed to specifically detect a cluster of this Rhodococcus group including isolated Rhodococcus strains, Rhodococcus opacus and Rhodococcus koreensis. The growth of this bacterial group in an o-xylene-contaminated soil was followed by competitive PCR (cPCR). The decrease in o-xylene clearly paralleled the growth of the Rhodococcus group.
|
| 巻・号 |
18(1)
|
| ページ |
17-26
|
| 公開日 |
2007-2-1
|
| DOI |
10.1007/s10532-005-9030-x
|
| PMID |
16485082
|
| MeSH |
Base Sequence
Biodegradation, Environmental
DNA Gyrase / genetics
DNA, Bacterial / genetics
DNA, Bacterial / isolation & purification
DNA, Ribosomal / genetics
DNA, Ribosomal / isolation & purification
Genes, Bacterial
Hydrocarbons, Aromatic / metabolism
Molecular Sequence Data
Phylogeny
Rhodococcus / classification
Rhodococcus / genetics
Rhodococcus / isolation & purification
Rhodococcus / metabolism*
Sequence Homology, Nucleic Acid
Soil Microbiology*
Soil Pollutants / metabolism*
Xylenes / metabolism*
|
| IF |
2.805
|
| 引用数 |
24
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 10743 |
