RRC ID 15415
著者 Pluemsab W, Fukazawa Y, Furuike T, Nodasaka Y, Sakairi N.
タイトル Cyclodextrin-linked alginate beads as supporting materials for Sphingomonas cloacae, a nonylphenol degrading bacteria.
ジャーナル Bioresour Technol
Abstract Calcium alginate beads covalently linked with alpha-cyclodextrin (alpha-CD-alginate beads) were prepared and examined for their ability to serve as a supporting matrix for bacterial degradation of nonylphenol, an endocrine disruptor. Column chromatographic experiment using alpha-CD-alginate beads with diameter of 657+/-82 microm and with degree of CD substitution of 0.16 showed a strong affinity for nonylphenol adsorption. Although addition of alpha-CD (2.7-27 mM) to the culture broth of Sphingomonas cloacae retarded nonylphenol degradation, the immobilized bacteria on the CD-alginate beads were effective for the degradation. Batch degradation tests using the immobilized bacteria on alpha-CD-alginate-beads showed 46% nonylphenol recovery after 10-day incubation at 25+/-2 degrees C, and the recovery reached to about 17% when wide and shallow incubation tubes were used to facilitate uptake of the viscous liquid of nonylphenol on the surface of the medium. Scanning electron microscopic photographs revealed that multiplicated bacteria was present both on the surface and inside the beads and the matrix of CD-alginate was stable and suitable during 10-day incubation.
巻・号 98(11)
ページ 2076-81
公開日 2007-8-1
DOI 10.1016/j.biortech.2006.08.009
PII S0960-8524(06)00416-0
PMID 17055261
MeSH Adsorption Alginates / chemistry* Biodegradation, Environmental Cyclodextrins / chemistry* Phenols / chemistry Phenols / metabolism* Sphingomonas / growth & development Sphingomonas / metabolism* Sphingomonas / ultrastructure Water Pollutants, Chemical / metabolism* Water Purification / methods
IF 7.539
引用数 20
WOS 分野 ENERGY & FUELS AGRICULTURAL ENGINEERING BIOTECHNOLOGY & APPLIED MICROBIOLOGY
リソース情報
一般微生物 JCM 10874