RRC ID 15419
Author Iwatani S, Zendo T, Yoneyama F, Nakayama J, Sonomoto K.
Title Characterization and structure analysis of a novel bacteriocin, lacticin Z, produced by Lactococcus lactis QU 14.
Journal Biosci Biotechnol Biochem
Abstract A novel bacteriocin, lacticin Z, produced by Lactococcus lactis QU 14 isolated from a horse's intestinal tract was identified. Lacticin Z was purified through a three step procedure comprised of hydrophobic-interaction, cation-exchange chromatography, and reverse-phase HPLC. ESI-TOF MS determined the molecular mass of lacticin Z to be 5,968.9 Da. The primary structure of lacticin Z was found to consist of 53 amino acid residues without any leader sequence or signal peptide. Lacticin Z showed homology to lacticin Q from L. lactis QU 5, aureocin A53 from Staphylococcus aureus A53, and mutacin BHT-B from Streptococcus rattus strain BHT. It exhibited a nanomolar range of MICs against various Gram-positive bacteria, and the activity was completely stable up to 100 degrees C. Unlike many of other LAB bacteriocins, the stability of lacticin Z was emphasized under alkaline conditions rather than acidic conditions. All the results indicated that lacticin Z belongs to a novel type of bacteriocin.
Volume 71(8)
Pages 1984-92
Published 2007-8-1
DOI 10.1271/bbb.70169
PII JST.JSTAGE/bbb/70169
PMID 17690480
MeSH Amino Acid Sequence Animals Bacterial Proteins Bacteriocins / chemistry Bacteriocins / isolation & purification Bacteriocins / pharmacology* Chromatography Gram-Positive Bacteria / drug effects Horses Lactococcus lactis / chemistry* Microbial Sensitivity Tests Sequence Homology, Amino Acid Spectrometry, Mass, Electrospray Ionization
IF 1.516
Times Cited 38
WOS Category FOOD SCIENCE & TECHNOLOGY CHEMISTRY, APPLIED BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
General Microbes JCM 1059 JCM 1070 JCM 1132 JCM 1134 JCM 1157 JCM 1164 JCM 1465 JCM 2504 JCM 2257 JCM 5705 JCM 5706 JCM 5707 JCM 5802 JCM 5885 JCM 5887 JCM 6124 JCM 7638 JCM 8797