Reference - Detail
| RRC ID | 15420 |
|---|---|
| Author | Nishimoto M, Kitaoka M. |
| Title | Practical preparation of lacto-N-biose I, a candidate for the bifidus factor in human milk. |
| Journal | Biosci Biotechnol Biochem |
| Abstract |
A one-pot enzymatic reaction to produce lacto-N-biose I (LNB), which is supposed to represent the bifidus factor in human milk oligosaccharides, was demonstrated. Approximately 500 mM of LNB was generated in 10-liter of reaction mixture initially containing 660 mM of sucrose and 600 mM of GlcNAc by the concurrent actions of four enzymes, sucrose phosphorylase, UDP-glucose-hexose-1-phospate uridylyltransferase, UDP-glucose 4-epimerase, and lacto-N-biose phosphorylase, in the presence of UDP-Glc and phosphate, indicating a reaction yield of 83%. LNB was isolated from the mixture by crystallization after yeast treatment. Finally, 1.4 kg of LNB of 99.6% purity was recovered after recrystallization. |
| Volume | 71(8) |
| Pages | 2101-4 |
| Published | 2007-8-1 |
| DOI | 10.1271/bbb.70320 |
| PII | JST.JSTAGE/bbb/70320 |
| PMID | 17690443 |
| MeSH | Acetylglucosamine / analogs & derivatives* Acetylglucosamine / chemical synthesis Crystallization Enzymes / metabolism Female Humans Milk, Human / chemistry* Molecular Sequence Data Oligosaccharides |
| IF | 1.516 |
| Times Cited | 98 |
| WOS Category | FOOD SCIENCE & TECHNOLOGY CHEMISTRY, APPLIED BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Resource | |
| General Microbes | JCM 1217 |