RRC ID 15423
Author Saegusa S, Totsuka M, Kaminogawa S, Hosoi T.
Title Cytokine responses of intestinal epithelial-like Caco-2 cells to non-pathogenic and opportunistic pathogenic yeasts in the presence of butyric acid.
Journal Biosci. Biotechnol. Biochem.
Abstract Candida albicans, Saccharomyces cerevisiae and their cell wall components, zymosan and glucan, have been shown to stimulate interleukin-8 (IL-8/CXCL-8) production by intestinal epithelial cell-like Caco-2 cells pre-cultured with 10 mM butyric acid. We examined in this study whether these yeasts also altered the production of other cytokines and cyclooxygenases (COXs) by Caco-2 cells. Culturing Caco-2 cells with 10 mM butyric acid and 15% FBS for 4 days enhanced the basal levels of mRNA encoding IL-6, IL-8, IL-18, monocyte chemoattractant protein (MCP)-1, stem cell factor, transforming growth factor (TGF)-beta1, TGF-beta3, tumor necrosis factor (TNF)-alpha, COX-1, and COX-2, but not of granulocyte-macrophage colony-stimulating factor (GM-CSF) and TGF-beta2. The inclusion of live S. cerevisiae or C. albicans further enhanced the production of IL-8, but not of the other cytokines and COXs. The non-pathogenic yeasts, C. kefyr, C. utilis, C. versatilis, Kluyveromyces lactis, K. marxianus, Schizosaccharomyces pombe and Zygosaccharomyces rouxii, used for the production of fermented foods and probiotics, and the opportunistic pathogens, C. glabrata, C. krusei, C. parapsilosis and C. tropicalis, isolated from human tissue samples also enhanced IL-8 secretion by Caco-2 cells.
Volume 71(10)
Pages 2428-34
Published 2007-10
DOI 10.1271/bbb.70172
PII JST.JSTAGE/bbb/70172
PMID 17928716
MeSH Butyric Acid / pharmacology* Caco-2 Cells Candida albicans / classification* Candida albicans / genetics Candida albicans / pathogenicity* Coculture Techniques Enzyme-Linked Immunosorbent Assay Epithelial Cells / immunology Epithelial Cells / microbiology Humans Interleukin-8 / biosynthesis* Interleukin-8 / genetics Intestines / cytology Intestines / drug effects* Intestines / immunology* Intestines / microbiology RNA, Messenger / genetics RNA, Messenger / metabolism Saccharomyces cerevisiae / pathogenicity* Time Factors
IF 1.255
Times Cited 16
WOS Category FOOD SCIENCE & TECHNOLOGY CHEMISTRY, APPLIED BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Human and Animal Cells
General Microbes JCM 1542 JCM 7255