論文 - 詳細
| RRC ID | 15427 |
|---|---|
| 著者 | Wamalwa BM, Zhao G, Sakka M, Shiundu PM, Kimura T, Sakka K. |
| タイトル | High-level heterologous expression of Bacillus halodurans putative xylanase xyn11a (BH0899) in Kluyveromyces lactis. |
| ジャーナル | Biosci Biotechnol Biochem |
| Abstract |
The putative xyn11A structural gene (BH0899) encoding a family-11 xylanase from alkaliphilic Bacillus halodurans strain C-125 was heterologously expressed in the yeast Kluyveromyces lactis CBS 1065 and secreted to a level of 156 microg/ml under selective culture conditions in shake flasks. The Xyn11A production level in shake flask cultures of K. lactis CBS 1065 was higher than that reported for other xylanase genes placed under the control of the regulated LAC4 promoter on a plasmid containing an entire sequence of pKD1 from Kluyveromyces drosophilarium. Recombinant Xyn11A was highly active over pH range from 3 to 10, with maximal activity around pH 7. The enzyme showed a specific activity of 628 U/mg-protein on birchwood xylan as substrate, but no cellulase or beta-xylosidase activity. |
| 巻・号 | 71(3) |
| ページ | 688-93 |
| 公開日 | 2007-3-1 |
| DOI | 10.1271/bbb.60477 |
| PII | JST.JSTAGE/bbb/60477 |
| PMID | 17341817 |
| MeSH | Bacillus / enzymology* Bacterial Proteins / biosynthesis* Bacterial Proteins / genetics Kluyveromyces / enzymology* Plasmids Promoter Regions, Genetic Recombinant Proteins / biosynthesis Recombinant Proteins / genetics Xylosidases / biosynthesis* Xylosidases / genetics |
| IF | 1.516 |
| 引用数 | 14 |
| WOS 分野 | FOOD SCIENCE & TECHNOLOGY CHEMISTRY, APPLIED BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| 一般微生物 | JCM 9153 |