Reference - Detail
| RRC ID | 15442 |
|---|---|
| Author | Morita T, Habe H, Fukuoka T, Imura T, Kitamoto D. |
| Title | Convenient transformation of anamorphic basidiomycetous yeasts belonging to genus pseudozyma induced by electroporation. |
| Journal | J Biosci Bioeng |
| Abstract |
A convenient procedure for carrying out transformation by electroporation was optimized for the genus Pseudozyma. Successful transformation was achieved using a plasmid, pUXV1, that confers resistance to hygromycin B; the maximum transformation efficiency was 48 transformants/mug of plasmid DNA. Transformants of Pseudozyma antarctica T-34 expressing a green fluorescent protein were obtained by the procedure. |
| Volume | 104(6) |
| Pages | 517-20 |
| Published | 2007-12-1 |
| DOI | 10.1263/jbb.104.517 |
| PII | S1389-1723(08)70013-6 |
| PMID | 18215641 |
| MeSH | Basidiomycota / classification* Basidiomycota / genetics* DNA, Fungal / genetics* Electroporation / methods* Gene Transfer Techniques* Plasmids / genetics* Transformation, Genetic / genetics* |
| IF | 2.366 |
| Times Cited | 15 |
| WOS Category | FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| Resource | |
| General Microbes | JCM 10317 JCM 10318 JCM 10323 |