論文 - 詳細
| RRC ID | 15470 |
|---|---|
| 著者 | Hollmann A, Delfederico L, Glikmann G, De Antoni G, Semorile L, Disalvo EA. |
| タイトル | Characterization of liposomes coated with S-layer proteins from lactobacilli. |
| ジャーナル | Biochim Biophys Acta |
| Abstract |
The stability of liposomes coated with S-layer proteins from Lactobacillus brevis and Lactobacillus kefir was analyzed as a previous stage to the development of a vaccine vehicle for oral administration. The interactions of the different S-layer proteins with positively charged liposomes prepared with soybean lecithin or dipalmitoylphosphatidylcholine were studied by means of the variation of the Z potential at different protein-lipid ratios, showing that both proteins were able to attach in a greater extent to the surface of soybean lecithin liposomes. The capacity of these particles to retain carboxyfluorescein or calcein by exposure to bile salts, pancreatic extract, pH change and after a thermal shock showed that both S-layer proteins increased the stability of the liposomes in the same magnitude. The non-glycosylated protein from L. brevis protects more efficiently the liposomes at pH 7 than those from L. kefir even without treatment with glutaraldehyde. |
| 巻・号 | 1768(3) |
| ページ | 393-400 |
| 公開日 | 2007-3-1 |
| DOI | 10.1016/j.bbamem.2006.09.009 |
| PII | S0005-2736(06)00353-1 |
| PMID | 17276386 |
| MeSH | Bacterial Proteins / chemistry* Bacterial Proteins / metabolism* Bile Acids and Salts / pharmacology Buffers Cross-Linking Reagents / pharmacology Fluoresceins / metabolism Fluorescent Dyes / metabolism Gastrointestinal Agents / pharmacology Glutaral / pharmacology Hot Temperature / adverse effects Hydrogen-Ion Concentration Lactobacillus / chemistry* Lactobacillus / genetics Liposomes / chemistry* Membrane Glycoproteins / chemistry* Membrane Glycoproteins / metabolism* Microscopy, Electron, Transmission Pancreatic Extracts / pharmacology |
| IF | 3.411 |
| 引用数 | 45 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 1059 JCM 5818 |