| RRC ID |
15470
|
| 著者 |
Hollmann A, Delfederico L, Glikmann G, De Antoni G, Semorile L, Disalvo EA.
|
| タイトル |
Characterization of liposomes coated with S-layer proteins from lactobacilli.
|
| ジャーナル |
Biochim Biophys Acta
|
| Abstract |
The stability of liposomes coated with S-layer proteins from Lactobacillus brevis and Lactobacillus kefir was analyzed as a previous stage to the development of a vaccine vehicle for oral administration. The interactions of the different S-layer proteins with positively charged liposomes prepared with soybean lecithin or dipalmitoylphosphatidylcholine were studied by means of the variation of the Z potential at different protein-lipid ratios, showing that both proteins were able to attach in a greater extent to the surface of soybean lecithin liposomes. The capacity of these particles to retain carboxyfluorescein or calcein by exposure to bile salts, pancreatic extract, pH change and after a thermal shock showed that both S-layer proteins increased the stability of the liposomes in the same magnitude. The non-glycosylated protein from L. brevis protects more efficiently the liposomes at pH 7 than those from L. kefir even without treatment with glutaraldehyde.
|
| 巻・号 |
1768(3)
|
| ページ |
393-400
|
| 公開日 |
2007-3-1
|
| DOI |
10.1016/j.bbamem.2006.09.009
|
| PII |
S0005-2736(06)00353-1
|
| PMID |
17276386
|
| MeSH |
Bacterial Proteins / chemistry*
Bacterial Proteins / metabolism*
Bile Acids and Salts / pharmacology
Buffers
Cross-Linking Reagents / pharmacology
Fluoresceins / metabolism
Fluorescent Dyes / metabolism
Gastrointestinal Agents / pharmacology
Glutaral / pharmacology
Hot Temperature / adverse effects
Hydrogen-Ion Concentration
Lactobacillus / chemistry*
Lactobacillus / genetics
Liposomes / chemistry*
Membrane Glycoproteins / chemistry*
Membrane Glycoproteins / metabolism*
Microscopy, Electron, Transmission
Pancreatic Extracts / pharmacology
|
| IF |
3.411
|
| 引用数 |
45
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 1059
JCM 5818 |
