論文 - 詳細
| RRC ID | 15570 |
|---|---|
| 著者 | Smith M, Mallin DR, Simon JA, Courey AJ. |
| タイトル | Small ubiquitin-like modifier (SUMO) conjugation impedes transcriptional silencing by the polycomb group repressor Sex Comb on Midleg. |
| ジャーナル | J Biol Chem |
| Abstract |
The Drosophila protein Sex Comb on Midleg (Scm) is a member of the Polycomb group (PcG), a set of transcriptional repressors that maintain silencing of homeotic genes during development. Recent findings have identified PcG proteins both as targets for modification by the small ubiquitin-like modifier (SUMO) protein and as catalytic components of the SUMO conjugation pathway. We have found that the SUMO-conjugating enzyme Ubc9 binds to Scm and that this interaction, which requires the Scm C-terminal sterile α motif (SAM) domain, is crucial for the efficient sumoylation of Scm. Scm is associated with the major Polycomb response element (PRE) of the homeotic gene Ultrabithorax (Ubx), and efficient PRE recruitment requires an intact Scm SAM domain. Global reduction of sumoylation augments binding of Scm to the PRE. This is likely to be a direct effect of Scm sumoylation because mutations in the SUMO acceptor sites in Scm enhance its recruitment to the PRE, whereas translational fusion of SUMO to the Scm N terminus interferes with this recruitment. In the metathorax, Ubx expression promotes haltere formation and suppresses wing development. When SUMO levels are reduced, we observe decreased expression of Ubx and partial haltere-to-wing transformation phenotypes. These observations suggest that SUMO negatively regulates Scm function by impeding its recruitment to the Ubx major PRE. |
| 巻・号 | 286(13) |
| ページ | 11391-400 |
| 公開日 | 2011-4-1 |
| DOI | 10.1074/jbc.M110.214569 |
| PII | S0021-9258(20)53762-9 |
| PMID | 21278366 |
| PMC | PMC3064195 |
| MeSH | Amino Acid Motifs Animals Binding Sites Cell Line Drosophila Proteins / biosynthesis* Drosophila Proteins / genetics Drosophila Proteins / metabolism Drosophila melanogaster Gene Silencing / physiology* Homeodomain Proteins / genetics Homeodomain Proteins / metabolism Polycomb-Group Proteins Repressor Proteins / biosynthesis* Repressor Proteins / genetics Response Elements / physiology SUMO-1 Protein / genetics SUMO-1 Protein / metabolism* Sumoylation / physiology* Transcription Factors / genetics Transcription Factors / metabolism Transcription, Genetic / physiology* Ubiquitin-Conjugating Enzymes / genetics Ubiquitin-Conjugating Enzymes / metabolism |
| IF | 4.238 |
| 引用数 | 22 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ショウジョウバエ | UAS-SUMORNAi 12359R-2 12359R-4 |