RRC ID 15571
Author Meghana C, Ramdas N, Hameed FM, Rao M, Shivashankar GV, Narasimha M.
Title Integrin adhesion drives the emergent polarization of active cytoskeletal stresses to pattern cell delamination.
Journal Proc Natl Acad Sci U S A
Abstract Tissue patterning relies on cellular reorganization through the interplay between signaling pathways and mechanical stresses. Their integration and spatiotemporal coordination remain poorly understood. Here we investigate the mechanisms driving the dynamics of cell delamination, diversely deployed to extrude dead cells or specify distinct cell fates. We show that a local mechanical stimulus (subcellular laser perturbation) releases cellular prestress and triggers cell delamination in the amnioserosa during Drosophila dorsal closure, which, like spontaneous delamination, results in the rearrangement of nearest neighbors around the delaminating cell into a rosette. We demonstrate that a sequence of "emergent cytoskeletal polarities" in the nearest neighbors (directed myosin flows, lamellipodial growth, polarized actomyosin collars, microtubule asters), triggered by the mechanical stimulus and dependent on integrin adhesion, generate active stresses that drive delamination. We interpret these patterns in the language of active gels as asters formed by active force dipoles involving surface and body stresses generated by each cell and liken delamination to mechanical yielding that ensues when these stresses exceed a threshold. We suggest that differential contributions of adhesion, cytoskeletal, and external stresses must underlie differences in spatial pattern.
Volume 108(22)
Pages 9107-12
Published 2011-5-31
DOI 10.1073/pnas.1018652108
PII 1018652108
PMID 21571643
PMC PMC3107263
MeSH Actomyosin / chemistry Animals Cell Adhesion Cell Lineage Cytoplasm / metabolism Cytoskeleton / metabolism* Drosophila Drosophila Proteins / metabolism* Gene Expression Regulation, Developmental* Green Fluorescent Proteins / metabolism Integrins / metabolism* Microscopy, Confocal / methods Models, Biological Time Factors Wound Healing
IF 9.58
Times Cited 22
WOS Category CELL BIOLOGY
Resource
Drosophila 1560R-1 1560R-2