| RRC ID |
15605
|
| Author |
Weelink SA, Tan NC, ten Broeke H, van den Kieboom C, van Doesburg W, Langenhoff AA, Gerritse J, Junca H, Stams AJ.
|
| Title |
Isolation and characterization of Alicycliphilus denitrificans strain BC, which grows on benzene with chlorate as the electron acceptor.
|
| Journal |
Appl Environ Microbiol
|
| Abstract |
A bacterium, strain BC, was isolated from a benzene-degrading chlorate-reducing enrichment culture. Strain BC degrades benzene in conjunction with chlorate reduction. Cells of strain BC are short rods that are 0.6 microm wide and 1 to 2 microm long, are motile, and stain gram negative. Strain BC grows on benzene and some other aromatic compounds with oxygen or in the absence of oxygen with chlorate as the electron acceptor. Strain BC is a denitrifying bacterium, but it is not able to grow on benzene with nitrate. The closest cultured relative is Alicycliphilus denitrificans type strain K601, a cyclohexanol-degrading nitrate-reducing betaproteobacterium. Chlorate reductase (0.4 U/mg protein) and chlorite dismutase (5.7 U/mg protein) activities in cell extracts of strain BC were determined. Gene sequences encoding a known chlorite dismutase (cld) were not detected in strain BC by using the PCR primers described in previous studies. As physiological and biochemical data indicated that there was oxygenation of benzene during growth with chlorate, a strategy was developed to detect genes encoding monooxygenase and dioxygenase enzymes potentially involved in benzene degradation in strain BC. Using primer sets designed to amplify members of distinct evolutionary branches in the catabolic families involved in benzene biodegradation, two oxygenase genes putatively encoding the enzymes performing the initial successive monooxygenations (BC-BMOa) and the cleavage of catechol (BC-C23O) were detected. Our findings suggest that oxygen formed by dismutation of chlorite can be used to attack organic molecules by means of oxygenases, as exemplified with benzene. Thus, aerobic pathways can be employed under conditions in which no external oxygen is supplied.
|
| Volume |
74(21)
|
| Pages |
6672-81
|
| Published |
2008-11-1
|
| DOI |
10.1128/AEM.00835-08
|
| PII |
AEM.00835-08
|
| PMID |
18791031
|
| PMC |
PMC2576721
|
| MeSH |
Bacterial Proteins / genetics
Bacterial Proteins / metabolism
Benzene / metabolism*
Chlorates / metabolism*
Comamonadaceae / classification*
Comamonadaceae / genetics
Comamonadaceae / isolation & purification*
Comamonadaceae / metabolism
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
DNA, Ribosomal / chemistry
DNA, Ribosomal / genetics
Dioxygenases / genetics
Dioxygenases / metabolism
Genes, rRNA
Locomotion
Mixed Function Oxygenases / genetics
Mixed Function Oxygenases / metabolism
Molecular Sequence Data
Nitrates / metabolism
Nitrites / metabolism
Nitrogen / metabolism
Oxidoreductases / genetics
Oxidoreductases / metabolism
Oxygen / metabolism
Phylogeny
Polymerase Chain Reaction / methods
RNA, Bacterial / genetics
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
|
| IF |
4.016
|
| Times Cited |
63
|
|
WOS Category
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
MICROBIOLOGY
|
| Resource |
| General Microbes |
JCM 14587 |
