| RRC ID |
15606
|
| 著者 |
Takano H, Shimizu A, Shibosawa R, Sasaki R, Iwagaki S, Minagawa O, Yamanaka K, Miwa K, Beppu T, Ueda K.
|
| タイトル |
Characterization of developmental colony formation in Corynebacterium glutamicum.
|
| ジャーナル |
Appl Microbiol Biotechnol
|
| Abstract |
We report that Corynebacterium glutamicum colonies exhibit a developmental transition in culture. When cultured on a routinely used complete medium (CM2B), this bacterium first formed a flat translucent colony. Subsequently, some parts of this colony expanded to form small spherical yellow colonies that finally developed into a single large yellow colony. The small flat colony consisted of long thick cells, which were occasionally V or Y shaped, while the large yellow colony consisted of short small rods. A similar colony development pattern was observed in Corynebacterium ammoniagenes and Corynebacterium callunae. Analysis following shotgun cloning revealed that the introduction of a multi-copy-number plasmid carrying amtR, a global transcriptional regulator for nitrogen metabolism, into C. glutamicum cells induced precocious colony development. An amtR-null C. glutamicum mutant exhibited delayed development. Detailed observations of C. glutamicum cells cultured on CM2B medium containing buffers at various pH values revealed that the colony growth was rapid at a pH value of 6.4 or higher and slow but distinct at a pH of less than 6.4. This pH threshold increased to 6.8 following the addition of 0.1% glucose into the medium.
|
| 巻・号 |
81(1)
|
| ページ |
127-34
|
| 公開日 |
2008-11-1
|
| DOI |
10.1007/s00253-008-1622-z
|
| PMID |
18696061
|
| MeSH |
Bacterial Proteins / genetics
Bacterial Proteins / metabolism
Corynebacterium glutamicum / cytology
Corynebacterium glutamicum / genetics
Corynebacterium glutamicum / growth & development*
Corynebacterium glutamicum / metabolism
Culture Media / chemistry
Culture Media / metabolism
Gene Expression
Glucose / metabolism
Quaternary Ammonium Compounds / metabolism
|
| IF |
3.53
|
| 引用数 |
2
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 1305
JCM 1318
JCM 9489 |
