Reference - Detail
| RRC ID | 15613 |
|---|---|
| Author | Ochou M, Saito M, Kurusu Y. |
| Title | Characterization of two compatible small plasmids from Sphingobium yanoikuyae. |
| Journal | Biosci Biotechnol Biochem |
| Abstract |
We isolated and characterized two small cryptic indigenous plasmids, pYAN-1 (4,896 bp) and pYAN-2 (4,687 bp), from Sphingobium yanoikuyae, and developed a versatile system that permitted genetic manipulation of the genus Sphingomonas. Nucleotide sequencing of both plasmids revealed that they contained mobA, mobs, and repA genes, which are predicted to encode proteins associated with mobilization and replication, in common. Transformation with each plasmid harboring the antibiotic resistance gene by electroporation was fully successful, using Novosphingobium capsulatum as a host. |
| Volume | 72(4) |
| Pages | 1130-3 |
| Published | 2008-4-1 |
| DOI | 10.1271/bbb.70813 |
| PII | JST.JSTAGE/bbb/70813 |
| PMID | 18391447 |
| MeSH | Open Reading Frames Plasmids / genetics* Sequence Analysis, DNA Sequence Homology, Amino Acid Sphingomonadaceae / genetics* |
| IF | 1.516 |
| Times Cited | 6 |
| WOS Category | FOOD SCIENCE & TECHNOLOGY CHEMISTRY, APPLIED BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Resource | |
| General Microbes | JCM 7371 JCM 7452 JCM 10275 JCM 10874 JCM 11777 |