論文 - 詳細
| RRC ID | 15613 |
|---|---|
| 著者 | Ochou M, Saito M, Kurusu Y. |
| タイトル | Characterization of two compatible small plasmids from Sphingobium yanoikuyae. |
| ジャーナル | Biosci Biotechnol Biochem |
| Abstract |
We isolated and characterized two small cryptic indigenous plasmids, pYAN-1 (4,896 bp) and pYAN-2 (4,687 bp), from Sphingobium yanoikuyae, and developed a versatile system that permitted genetic manipulation of the genus Sphingomonas. Nucleotide sequencing of both plasmids revealed that they contained mobA, mobs, and repA genes, which are predicted to encode proteins associated with mobilization and replication, in common. Transformation with each plasmid harboring the antibiotic resistance gene by electroporation was fully successful, using Novosphingobium capsulatum as a host. |
| 巻・号 | 72(4) |
| ページ | 1130-3 |
| 公開日 | 2008-4-1 |
| DOI | 10.1271/bbb.70813 |
| PII | JST.JSTAGE/bbb/70813 |
| PMID | 18391447 |
| MeSH | Open Reading Frames Plasmids / genetics* Sequence Analysis, DNA Sequence Homology, Amino Acid Sphingomonadaceae / genetics* |
| IF | 1.516 |
| 引用数 | 6 |
| WOS 分野 | FOOD SCIENCE & TECHNOLOGY CHEMISTRY, APPLIED BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| 一般微生物 | JCM 7371 JCM 7452 JCM 10275 JCM 10874 JCM 11777 |