| RRC ID |
15618
|
| 著者 |
Tagawa M, Tamaki H, Manome A, Koyama O, Kamagata Y.
|
| タイトル |
Development of a genotyping method for potato scab pathogens based on multiplex PCR.
|
| ジャーナル |
Biosci Biotechnol Biochem
|
| Abstract |
Scab disease significantly damages potato and other root crops. Streptomyces scabiei, S. acidiscabiei, and S. turgidiscabiei are the best-known causal agents of this disease. We have developed a novel genotyping method for these potato scab pathogens using multiplex PCR, whose benefits include rapid and easy detection of multiple species. We designed a species-specific primer set (6 primers, 3 pairs) for the 16S rRNA genes and 16S-23S ITS regions of these potato scab pathogens. The specificity of the primer set was confirmed by testing 18 strains containing potato scab pathogens, other Streptomyces species, and strains of other genera. The application of the developed method to potato field soil and potato tissue samples resulted in the clear detection and identification of pathogens. Since this method is applicable to a large number of environmental samples, it is expected to be useful for a high-throughput analysis of soil and plant tissues of scab disease.
|
| 巻・号 |
72(9)
|
| ページ |
2324-34
|
| 公開日 |
2008-9-1
|
| DOI |
10.1271/bbb.80234
|
| PII |
JST.JSTAGE/bbb/80234
|
| PMID |
18776692
|
| MeSH |
Animals
DNA Primers / genetics
DNA, Bacterial / analysis
DNA, Bacterial / genetics
DNA, Ribosomal / genetics
DNA, Ribosomal Spacer / genetics
Genes, rRNA
Genetic Techniques*
Phylogeny
Plant Diseases / genetics*
Polymerase Chain Reaction / methods*
RNA, Bacterial / genetics
RNA, Ribosomal, 16S / genetics
RNA, Ribosomal, 23S / genetics
Sensitivity and Specificity
Solanum tuberosum / genetics*
Streptomyces / classification
Streptomyces / genetics*
|
| IF |
1.516
|
| 引用数 |
8
|
|
WOS 分野
|
FOOD SCIENCE & TECHNOLOGY
CHEMISTRY, APPLIED
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 7913
JCM 7914
JCM 10429 |
