RRC ID 15622
Author Iijima K, Asano S, Suzuki K, Ogata T, Kitagawa Y.
Title Modified multiplex PCR methods for comprehensive detection of Pectinatus and beer-spoilage cocci.
Journal Biosci Biotechnol Biochem
Abstract Specific PCR primers were designed based on the 16S rRNA genes of recently proposed beer-spoilage species, Pectinatus haikarae, Megasphaera sueciensis, and M. paucivorans, and two sets of our previously reported multiplex PCR methods for Pectinatus spp. and beer-spoilage cocci were reconstructed. Each modified multiplex PCR method was found specifically to detect beer-spoilage species of Pectinatus and cocci, including new species.
Volume 72(10)
Pages 2764-6
Published 2008-10-1
DOI 10.1271/bbb.80297
PII JST.JSTAGE/bbb/80297
PMID 18838790
MeSH Beer* Megasphaera / genetics* Pectinatus / genetics* Polymerase Chain Reaction / methods* Sensitivity and Specificity
IF 1.516
Times Cited 16
WOS Category FOOD SCIENCE & TECHNOLOGY CHEMISTRY, APPLIED BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
General Microbes JCM 5805 JCM 5885 JCM 5886 JCM 5887 JCM 5889 JCM 5890 JCM 6124