論文 - 詳細
| RRC ID | 15624 |
|---|---|
| 著者 | Okumura T, Nagai F, Yamamoto S, Hashimoto T, Ito M, Sawada H. |
| タイトル | Development of the reverse passive latex agglutination method for the detection and quantification of the genus Nitrospira in the wastewater treatment process. |
| ジャーナル | Biosci Biotechnol Biochem |
| Abstract |
This report describes a new immunological method for the detection and quantification of Nitrospira populations using the reverse passive latex agglutination (RPLA). The numbers of the genus Nitrospira have been quantified only by molecular biological techniques such as FISH and quantitative PCR to date. Using high-density latex particles and a specific polyclonal antibody, Nitrospira populations in the wastewater treatment process were quantified in the shortest 4 h of incubation. The minimum detectable number of Nitrospira cells was 7.0x10(5) (log(10) 5.85) cells/ml. It is thought that the RPLA method can quantify Nitrospira populations more simply, economically, and speedily than molecular biological techniques or the culture method, because this procedure has a simple protocol and does not require the use of specialized equipment, expensive reagents, or technical skill. Therefore it is applicable for use in the everyday control and maintenance of water quality in wastewater treatment facilities where equipment is not sufficient or in the field. |
| 巻・号 | 72(2) |
| ページ | 360-7 |
| 公開日 | 2008-2-1 |
| DOI | 10.1271/bbb.70427 |
| PII | JST.JSTAGE/bbb/70427 |
| PMID | 18256494 |
| MeSH | Bacteria / genetics Bacteria / isolation & purification* Base Sequence DNA Primers In Situ Hybridization, Fluorescence Latex Fixation Tests / methods* Polymerase Chain Reaction Water Microbiology* |
| IF | 1.516 |
| 引用数 | 0 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY CHEMISTRY, APPLIED FOOD SCIENCE & TECHNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 3870 JCM 5969 |