| RRC ID |
15636
|
| 著者 |
Voordeckers JW, Do MH, Hügler M, Ko V, Sievert SM, Vetriani C.
|
| タイトル |
Culture dependent and independent analyses of 16S rRNA and ATP citrate lyase genes: a comparison of microbial communities from different black smoker chimneys on the Mid-Atlantic Ridge.
|
| ジャーナル |
Extremophiles
|
| Abstract |
The bacterial and archaeal communities of three deep-sea hydrothermal vent systems located on the Mid-Atlantic Ridge (MAR; Rainbow, Logatchev and Broken Spur) were investigated using an integrated culture-dependent and independent approach. Comparative molecular phylogenetic analyses, using the 16S rRNA gene and the deduced amino acid sequences of the alpha and beta subunits of the ATP citrate lyase encoding genes were carried out on natural microbial communities, on an enrichment culture obtained from the Broken Spur chimney, and on novel chemolithoautotrophic bacteria and reference strains originally isolated from several different deep-sea vents. Our data showed that the three MAR hydrothermal vent chimneys investigated in this study host very different microbial assemblages. The microbial community of the Rainbow chimney was dominated by thermophilic, autotrophic, hydrogen-oxidizing, sulfur- and nitrate-reducing Epsilonproteobacteria related to the genus Caminibacter. The detection of sequences related to sulfur-reducing bacteria and archaea (Archaeoglobus) indicated that thermophilic sulfate reduction might also be occurring at this site. The Logatchev bacterial community included several sequences related to mesophilic sulfur-oxidizing bacteria, while the archaeal component of this chimney was dominated by sequences related to the ANME-2 lineage, suggesting that anaerobic oxidation of methane may be occurring at this site. Comparative analyses of the ATP citrate lyase encoding genes from natural microbial communities suggested that Epsilonproteobacteria were the dominant primary producers using the reverse TCA cycle (rTCA) at Rainbow, while Aquificales of the genera Desulfurobacterium and Persephonella were prevalent in the Broken Spur chimney.
|
| 巻・号 |
12(5)
|
| ページ |
627-40
|
| 公開日 |
2008-9-1
|
| DOI |
10.1007/s00792-008-0167-5
|
| PMID |
18523725
|
| MeSH |
ATP Citrate (pro-S)-Lyase / genetics*
Alphaproteobacteria / enzymology
Alphaproteobacteria / genetics
Atlantic Ocean
Bacteria / classification
Bacteria / enzymology
Bacteria / genetics*
Bacteria / isolation & purification
DNA, Bacterial / genetics
DNA, Bacterial / isolation & purification
Epsilonproteobacteria / enzymology
Epsilonproteobacteria / genetics
Gammaproteobacteria / enzymology
Gammaproteobacteria / genetics
Geography
Hydrogen / metabolism
Hydrogen-Ion Concentration
Oxidation-Reduction
Phylogeny
RNA, Bacterial / genetics
RNA, Ribosomal, 16S / genetics*
Seawater / microbiology*
Water Microbiology*
|
| IF |
2.462
|
| 引用数 |
36
|
|
WOS 分野
|
MICROBIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 11971
JCM 12117 |
