| RRC ID |
15648
|
| Author |
Hussain M, von Eiff C, Sinha B, Joost I, Herrmann M, Peters G, Becker K.
|
| Title |
eap Gene as novel target for specific identification of Staphylococcus aureus.
|
| Journal |
J Clin Microbiol
|
| Abstract |
The cell surface-associated extracellular adherence protein (Eap) mediates adherence of Staphylococcus aureus to host extracellular matrix components and inhibits inflammation, wound healing, and angiogenesis. A well-characterized collection of S. aureus and non-S. aureus staphylococcal isolates (n = 813) was tested for the presence of the Eap-encoding gene (eap) by PCR to investigate the use of the eap gene as a specific diagnostic tool for identification of S. aureus. Whereas all 597 S. aureus isolates were eap positive, this gene was not detectable in 216 non-S. aureus staphylococcal isolates comprising 47 different species and subspecies of coagulase-negative staphylococci and non-S. aureus coagulase-positive or coagulase-variable staphylococci. Furthermore, non-S. aureus isolates did not express Eap homologs, as verified on the transcriptional and protein levels. Based on these data, the sensitivity and specificity of the newly developed PCR targeting the eap gene were both 100%. Thus, the unique occurrence of Eap in S. aureus offers a promising tool particularly suitable for molecular diagnostics of this pathogen.
|
| Volume |
46(2)
|
| Pages |
470-6
|
| Published |
2008-2-1
|
| DOI |
10.1128/JCM.01425-07
|
| PII |
JCM.01425-07
|
| PMID |
18094124
|
| PMC |
PMC2238111
|
| MeSH |
Bacterial Proteins / analysis
Bacterial Proteins / genetics*
Blotting, Northern
Blotting, Western
DNA, Bacterial / genetics
Electrophoresis, Polyacrylamide Gel
Humans
Polymerase Chain Reaction / methods*
RNA-Binding Proteins / analysis
RNA-Binding Proteins / genetics*
Sensitivity and Specificity
Staphylococcal Infections / diagnosis*
Staphylococcal Infections / microbiology
Staphylococcus aureus / chemistry
Staphylococcus aureus / genetics
Staphylococcus aureus / isolation & purification*
|
| IF |
5.897
|
| Times Cited |
40
|
|
WOS Category
|
MICROBIOLOGY
|
| Resource |
| General Microbes |
JCM 11467
JCM 13363
JCM 14000 |
