論文 - 詳細
| RRC ID | 15659 |
|---|---|
| 著者 | Fujii K, Matsumoto HN, Koyama Y, Iwasaki Y, Ishihara K, Takakuda K. |
| タイトル | Prevention of biofilm formation with a coating of 2-methacryloyloxyethyl phosphorylcholine polymer. |
| ジャーナル | J Vet Med Sci |
| Abstract |
Device-associated infections are serious complications, and their prevention is an issue of considerable importance. Since biofilms are responsible for these refractory infections, effective methods to inhibit biofilm formation are required. In this investigation, stainless steel plates with and without 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer, i.e., poly (MPC-co-n-butyl methacrylate) (PMB) coating, were incubated in a medium containing bacteria. In the course of incubation, half of the specimens received antibiotics. The specimens were stained for nucleic acid and polysaccharides, and then examined with a confocal laser scanning microscope. The numbers of bacteria on the specimen surfaces were evaluated by an ATP assay. On the surfaces of the specimens without PMB coating, the formation of a biofilm enveloping bacteria was confirmed. The addition of antibiotics did not effectively decrease the number of bacteria. On the other hand, on the surfaces of the specimens with PMB coating, no biofilm formation was observed, and the number of bacteria was significantly decreased. The addition of potent antibiotics further decreased the number of bacteria by 1/100 to 1/1000 times. The PMB coating combined with the validated use of antibiotics might provide a method for the simultaneous achievement of biocompatible surfaces of devices and the prevention of device-associated infections. |
| 巻・号 | 70(2) |
| ページ | 167-73 |
| 公開日 | 2008-2-1 |
| DOI | 10.1292/jvms.70.167 |
| PII | JST.JSTAGE/jvms/70.167 |
| PMID | 18319577 |
| MeSH | Anti-Bacterial Agents / chemistry Anti-Bacterial Agents / pharmacology* Bacteria / drug effects* Bacteria / growth & development* Biofilms / drug effects* Biofilms / growth & development* Coated Materials, Biocompatible Materials Testing Methacrylates / chemistry Methacrylates / pharmacology* Phosphorylcholine / analogs & derivatives* Phosphorylcholine / chemistry Phosphorylcholine / pharmacology Polymers / chemistry Polymers / pharmacology Stainless Steel / chemistry* |
| IF | 1.049 |
| 引用数 | 35 |
| WOS 分野 | VETERINARY SCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 2151 JCM 2412 JCM 2414 |