RRC ID 157
著者 Ide M, Kuwamura M, Kotani T, Sawamoto O, Yamate J.
タイトル Effects of gadolinium chloride (GdCl(3)) on the appearance of macrophage populations and fibrogenesis in thioacetamide-induced rat hepatic lesions.
ジャーナル J Comp Pathol
Abstract Macrophages infiltrating injured tissue play an important part in fibrogenesis. To shed light on the functional roles of macrophages, we investigated the appearance of macrophage populations in thioacetamide (TAA)-induced rat hepatic lesions, with or without pretreatment with GdCl(3), a chemical capable of inhibiting Kupffer cell functions. In the GdCl(3)+TAA group rats received a single intraperitoneal injection of GdCl(3) (7.5mg/kg body weight) and, after 24h, a single intravenous injection of TAA (300mg/kg body weight). Rats in the TAA group received TAA only. Rats in both groups were examined on post-TAA injection (PTI) days 3, 5, and 7. In the TAA group, on PTI day 3, when TAA-induced hepatocyte injury was particularly prominent, the number of macrophages peaked, subsequently decreasing until PTI day 7. As compared with the TAA group, the GdCl(3)+TAA group showed significantly decreased numbers of ED1-immunolabelled cells (exudate macrophages) and ED2-immunolabelled cells (Kupffer cells) on PTI days 3, 5, and 7, and OX6-immunolabelled cells (antigen-presenting macrophages) on PTI days 3 and 5. Although less strikingly, the numbers of alpha-smooth muscle actin-positive myofibroblasts and fibrotic areas were decreased in the GdCl(3)+TAA group. By RT-PCR, the expression of TGF-beta1 mRNA was suppressed on PTI days 3 and 7 in the GdCl(3)+TAA group, and the suppressed expression was confirmed in vitro by treating rat macrophage-like cells (HS-P) with 1% GdCl(3). The study showed that GdCl(3) treatment decreased the numbers of macrophages appearing in hepatic lesions and inhibited TGF-beta1 mRNA expression in macrophages. Decreased numbers of macrophages may contribute to improvement of hepatic fibrosis.
巻・号 133(2-3)
ページ 92-102
公開日 2005-1-1
DOI 10.1016/j.jcpa.2005.01.011
PII S0021-9975(05)00028-9
PMID 15964588
MeSH Alanine Transaminase / blood Animals Anti-Inflammatory Agents / therapeutic use* Aspartate Aminotransferases / blood Biomarkers / metabolism Cell Survival / drug effects Disease Models, Animal Gadolinium / therapeutic use* Gene Expression Regulation / drug effects Hepatocytes / drug effects Hepatocytes / metabolism Injections, Intraperitoneal Injections, Intravenous Liver Cirrhosis / drug therapy* Liver Cirrhosis / metabolism Liver Cirrhosis / pathology Macrophages / drug effects* Male RNA, Messenger / metabolism Rats Rats, Inbred F344 Reverse Transcriptase Polymerase Chain Reaction Thioacetamide / toxicity* Transforming Growth Factor beta / genetics Transforming Growth Factor beta / metabolism Transforming Growth Factor beta1 Tumor Cells, Cultured / drug effects Tumor Cells, Cultured / pathology
IF 0.994
引用数 62
WOS 分野 VETERINARY SCIENCES PATHOLOGY
リソース情報
ラット F344/DuCrlCrlj(strainID=537)