論文 - 詳細
| RRC ID | 157 |
|---|---|
| 著者 | Ide M, Kuwamura M, Kotani T, Sawamoto O, Yamate J. |
| タイトル | Effects of gadolinium chloride (GdCl(3)) on the appearance of macrophage populations and fibrogenesis in thioacetamide-induced rat hepatic lesions. |
| ジャーナル | J Comp Pathol |
| Abstract |
Macrophages infiltrating injured tissue play an important part in fibrogenesis. To shed light on the functional roles of macrophages, we investigated the appearance of macrophage populations in thioacetamide (TAA)-induced rat hepatic lesions, with or without pretreatment with GdCl(3), a chemical capable of inhibiting Kupffer cell functions. In the GdCl(3)+TAA group rats received a single intraperitoneal injection of GdCl(3) (7.5mg/kg body weight) and, after 24h, a single intravenous injection of TAA (300mg/kg body weight). Rats in the TAA group received TAA only. Rats in both groups were examined on post-TAA injection (PTI) days 3, 5, and 7. In the TAA group, on PTI day 3, when TAA-induced hepatocyte injury was particularly prominent, the number of macrophages peaked, subsequently decreasing until PTI day 7. As compared with the TAA group, the GdCl(3)+TAA group showed significantly decreased numbers of ED1-immunolabelled cells (exudate macrophages) and ED2-immunolabelled cells (Kupffer cells) on PTI days 3, 5, and 7, and OX6-immunolabelled cells (antigen-presenting macrophages) on PTI days 3 and 5. Although less strikingly, the numbers of alpha-smooth muscle actin-positive myofibroblasts and fibrotic areas were decreased in the GdCl(3)+TAA group. By RT-PCR, the expression of TGF-beta1 mRNA was suppressed on PTI days 3 and 7 in the GdCl(3)+TAA group, and the suppressed expression was confirmed in vitro by treating rat macrophage-like cells (HS-P) with 1% GdCl(3). The study showed that GdCl(3) treatment decreased the numbers of macrophages appearing in hepatic lesions and inhibited TGF-beta1 mRNA expression in macrophages. Decreased numbers of macrophages may contribute to improvement of hepatic fibrosis. |
| 巻・号 | 133(2-3) |
| ページ | 92-102 |
| 公開日 | 2005-1-1 |
| DOI | 10.1016/j.jcpa.2005.01.011 |
| PII | S0021-9975(05)00028-9 |
| PMID | 15964588 |
| MeSH | Alanine Transaminase / blood Animals Anti-Inflammatory Agents / therapeutic use* Aspartate Aminotransferases / blood Biomarkers / metabolism Cell Survival / drug effects Disease Models, Animal Gadolinium / therapeutic use* Gene Expression Regulation / drug effects Hepatocytes / drug effects Hepatocytes / metabolism Injections, Intraperitoneal Injections, Intravenous Liver Cirrhosis / drug therapy* Liver Cirrhosis / metabolism Liver Cirrhosis / pathology Macrophages / drug effects* Male RNA, Messenger / metabolism Rats Rats, Inbred F344 Reverse Transcriptase Polymerase Chain Reaction Thioacetamide / toxicity* Transforming Growth Factor beta / genetics Transforming Growth Factor beta / metabolism Transforming Growth Factor beta1 Tumor Cells, Cultured / drug effects Tumor Cells, Cultured / pathology |
| IF | 0.994 |
| 引用数 | 62 |
| WOS 分野 | VETERINARY SCIENCES PATHOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ラット | F344/DuCrlCrlj(strainID=537) |