| RRC ID |
15708
|
| 著者 |
Kawai Y, Kusnadi J, Kemperman R, Kok J, Ito Y, Endo M, Arakawa K, Uchida H, Nishimura J, Kitazawa H, Saito T.
|
| タイトル |
DNA sequencing and homologous expression of a small peptide conferring immunity to gassericin A, a circular bacteriocin produced by Lactobacillus gasseri LA39.
|
| ジャーナル |
Appl Environ Microbiol
|
| Abstract |
Gassericin A, produced by Lactobacillus gasseri LA39, is a hydrophobic circular bacteriocin. The DNA region surrounding the gassericin A structural gene, gaaA, was sequenced, and seven open reading frames (ORFs) of 3.5 kbp (gaaBCADITE) were found with possible functions in gassericin A production, secretion, and immunity. The deduced products of the five consecutive ORFs gaaADITE have homology to those of genes involved in butyrivibriocin AR10 production, although the genetic arrangements are different in the two circular bacteriocin genes. GaaI is a small, positively charged hydrophobic peptide of 53 amino acids containing a putative transmembrane segment. Heterologous expression and homologous expression of GaaI in Lactococcus lactis subsp. cremoris MG1363 and L. gasseri JCM1131(T), respectively, were studied. GaaI-expressing strains exhibited at least sevenfold-higher resistance to gassericin A than corresponding control strains, indicating that gaaI encodes an immunity peptide for gassericin A. Comparison of GaaI to peptides with similar characteristics found in the circular bacteriocin gene loci is discussed.
|
| 巻・号 |
75(5)
|
| ページ |
1324-30
|
| 公開日 |
2009-3-1
|
| DOI |
10.1128/AEM.02485-08
|
| PII |
AEM.02485-08
|
| PMID |
19114506
|
| PMC |
PMC2648179
|
| MeSH |
Amino Acid Sequence
Anti-Bacterial Agents / antagonists & inhibitors*
Anti-Bacterial Agents / pharmacology
Bacterial Proteins / genetics*
Bacterial Proteins / metabolism
Bacteriocins / antagonists & inhibitors*
Bacteriocins / pharmacology
Cloning, Molecular
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
Drug Resistance, Bacterial*
Gene Order
Lactobacillus / drug effects*
Lactobacillus / genetics
Metabolic Networks and Pathways
Molecular Sequence Data
Open Reading Frames
Sequence Analysis, DNA
Sequence Homology, Amino Acid
|
| IF |
4.016
|
| 引用数 |
23
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 1131
JCM 11657 |
