| RRC ID |
15710
|
| Author |
Nakajima M, Nishimoto M, Kitaoka M.
|
| Title |
Characterization of beta-1,3-galactosyl-N-acetylhexosamine phosphorylase from Propionibacterium acnes.
|
| Journal |
Appl Microbiol Biotechnol
|
| Abstract |
Homologs of the beta-1,3-galactosyl-N-acetylhexosamine phosphorylase (GalHexNAcP) gene (gnpA) were cloned from the genomic DNA of Propionibacterium acnes JCM6425 and P. acnes JCM6473, showing 99.9% and 97.9% nucleotide sequence identity, respectively, with the ppa0083 gene from the genome-sequenced P. acnes KPA171202. No gnpA gene was detected in the genomic DNA of type strain P. acnes ATCC25746. The recombinant enzyme from P. acnes JCM6425 (GnpA) showed approximately 70 times higher specific activity of phosphorolysis on galacto-N-biose (Galbeta1-->3GalNAc, GNB) than that on lacto-N-biose I (Galbeta1-->3GlcNAc). K (m) value for GnpA on GNB was high, but GnpA did not exhibit activity on any derivatives of GNB examined. These results indicate that GnpA is GalHexNAcP which should be classified as galacto-N-biose phosphorylase. The large k (cat) value of GnpA on GalNAc suggests that GnpA would be a useful catalyst for the synthesis of GNB.
|
| Volume |
83(1)
|
| Pages |
109-15
|
| Published |
2009-5-1
|
| DOI |
10.1007/s00253-008-1838-y
|
| PMID |
19132369
|
| MeSH |
Acetylglucosamine / analogs & derivatives
Acetylglucosamine / metabolism
Cloning, Molecular
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
Disaccharides / metabolism
Enzyme Stability
Galactosyltransferases / genetics*
Galactosyltransferases / metabolism*
Hydrogen-Ion Concentration
Kinetics
Molecular Sequence Data
Phylogeny
Propionibacterium acnes / enzymology*
Propionibacterium acnes / genetics
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
Substrate Specificity
Temperature
|
| IF |
3.53
|
| Times Cited |
15
|
|
WOS Category
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
| Resource |
| General Microbes |
JCM 6425
JCM 6473 |
