RRC ID 15716
Author Miyake Y, Ito C, Itoigawa M, Osawa T.
Title Antioxidants produced by Eurotium herbariorum of filamentous fungi used for the manufacture of karebushi, dried bonito (Katsuobushi).
Journal Biosci Biotechnol Biochem
Abstract Extracts prepared by culturing ten filamentous fungi from Aspergillus and Eurotium species isolated from dried bonito (katsuobushi) were examined for 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capacity. The extracts prepared by culturing E. herbariorum NE-1 and NE-4, which are used in the molding process for the manufacture of karebushi (a kind of katsuobushi), were shown to have higher activity than the others. Five antioxidants were isolated from the extracts and identified as isodihydroauroglaucin (IDAG), auroglaucin (AG), dihydroauroglaucin (DAG), tetrahydroauroglaucin (TAG), and flavoglaucin (FG) by (1)H-NMR, (13)C-NMR, and EI-MS analyses. Compared with alpha-tocopherol, the isolated antioxidants exhibited high antioxidative activity for the radical scavenging capacity of DPPH and superoxide, but low activity for inhibiting the autoxidation of docosahexaenoic acid (DHA). The isolated antioxidants were produced by the Eurotium species, but not by the Aspergillus species. DAG and TAG exhibited higher radical scavenging capacity than the other antioxidants and were abundantly contained in the extracts of E. herbariorum NE-1 and NE-4.
Volume 73(6)
Pages 1323-7
Published 2009-6-1
DOI 10.1271/bbb.80887
PII JST.JSTAGE/bbb/80887
PMID 19502740
MeSH Antioxidants / chemistry* Antioxidants / metabolism Antioxidants / pharmacology Aspergillus / metabolism* Chromatography, High Pressure Liquid Eurotium / metabolism* Free Radical Scavengers / chemistry Free Radical Scavengers / metabolism Free Radical Scavengers / pharmacology Magnetic Resonance Spectroscopy Spectrometry, Mass, Electrospray Ionization
IF 1.516
Times Cited 20
WOS Category FOOD SCIENCE & TECHNOLOGY CHEMISTRY, APPLIED BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
General Microbes JCM 23047 JCM 23060