RRC ID 15722
Author Yamada K, Sato K, Morishita S, Kaminogawa S, Totsuka M.
Title Establishment of a primary culture method for mouse intestinal epithelial cells by organ culture of fetal small intestine.
Journal Biosci Biotechnol Biochem
Abstract Studies of the physiological functions of intestinal epithelial cells (IECs) have been limited by the difficulty of primary culture of IEC. We established a method for primary culture of mouse IEC by culturing fragments of fetal small intestines pretreated with EDTA. This method reproducibly resulted in the expansion of cytokeratin-positive epithelial cells, and vigorous expansion of the epithelial cells was observed only from intestinal fragments of embryonic days 15-16. These cells expressed alkaline phosphatase activity and major histocompatibility complex (MHC) class II molecules, indicating the mature phenotype of IEC in a small intestine. The cells also presented antigens to CD4(+) T cells. Furthermore, the cells expressed various cytokines and chemokines, and the expression was enhanced by bacterial stimulation. These results indicate that the primary-cultured mouse IEC prepared by the method established here can be a beneficial tool in study of the functions of IECs, especially in mucosal immunity.
Volume 73(8)
Pages 1849-55
Published 2009-8-1
DOI 10.1271/bbb.90246
PII JST.JSTAGE/bbb/90246
PMID 19661685
MeSH Animals Antigen Presentation Biomarkers CD4-Positive T-Lymphocytes / immunology Cell Culture Techniques / methods* Cell Proliferation Chemokines / genetics Epithelial Cells / cytology* Epithelial Cells / immunology Escherichia coli / immunology Female Fetus* Gene Expression Regulation / immunology Intestine, Small* Lipopolysaccharides / immunology Mice Mice, Inbred BALB C Organ Culture Techniques Peptides / immunology Pregnancy
IF 1.516
Times Cited 9
WOS Category FOOD SCIENCE & TECHNOLOGY CHEMISTRY, APPLIED BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
General Microbes JCM 1649