RRC ID |
15722
|
著者 |
Yamada K, Sato K, Morishita S, Kaminogawa S, Totsuka M.
|
タイトル |
Establishment of a primary culture method for mouse intestinal epithelial cells by organ culture of fetal small intestine.
|
ジャーナル |
Biosci Biotechnol Biochem
|
Abstract |
Studies of the physiological functions of intestinal epithelial cells (IECs) have been limited by the difficulty of primary culture of IEC. We established a method for primary culture of mouse IEC by culturing fragments of fetal small intestines pretreated with EDTA. This method reproducibly resulted in the expansion of cytokeratin-positive epithelial cells, and vigorous expansion of the epithelial cells was observed only from intestinal fragments of embryonic days 15-16. These cells expressed alkaline phosphatase activity and major histocompatibility complex (MHC) class II molecules, indicating the mature phenotype of IEC in a small intestine. The cells also presented antigens to CD4(+) T cells. Furthermore, the cells expressed various cytokines and chemokines, and the expression was enhanced by bacterial stimulation. These results indicate that the primary-cultured mouse IEC prepared by the method established here can be a beneficial tool in study of the functions of IECs, especially in mucosal immunity.
|
巻・号 |
73(8)
|
ページ |
1849-55
|
公開日 |
2009-8-1
|
DOI |
10.1271/bbb.90246
|
PII |
JST.JSTAGE/bbb/90246
|
PMID |
19661685
|
MeSH |
Animals
Antigen Presentation
Biomarkers
CD4-Positive T-Lymphocytes / immunology
Cell Culture Techniques / methods*
Cell Proliferation
Chemokines / genetics
Epithelial Cells / cytology*
Epithelial Cells / immunology
Escherichia coli / immunology
Female
Fetus*
Gene Expression Regulation / immunology
Intestine, Small*
Lipopolysaccharides / immunology
Mice
Mice, Inbred BALB C
Organ Culture Techniques
Peptides / immunology
Pregnancy
|
IF |
1.516
|
引用数 |
9
|
WOS 分野
|
FOOD SCIENCE & TECHNOLOGY
CHEMISTRY, APPLIED
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
一般微生物 |
JCM 1649 |