| RRC ID |
15725
|
| 著者 |
Iida T, Moteki Y, Nakamura K, Taguchi K, Otagiri M, Asanuma M, Dohmae N, Usami R, Kudo T.
|
| タイトル |
Functional expression of three Rieske non-heme iron oxygenases derived from actinomycetes in Rhodococcus species for investigation of their degradation capabilities of dibenzofuran and chlorinated dioxins.
|
| ジャーナル |
Biosci Biotechnol Biochem
|
| Abstract |
The activity of Rieske non-heme iron oxygenases (aromatic hydrocarbon dioxygenases, AhDOs) is important for the bacterial degradation of aromatic pollutants such as polycyclic aromatic hydrocarbons and dioxins. During our analysis of the role of AhDOs in dioxin bioremediation, some enzymes derived from high G + C Gram-positive actinomycetes were difficult to produce in active form in the Escherichia coli protein expression system. In this study, we constructed a heterologous expression system for AhDOs in Rhodococcus species using a constitutive expression promoter, P(dfdB), and a shuttle vector, pRK401, and analyzed the ability of these enzymes to degrade dibenzofuran and deplete several chlorinated dioxins. Three active AhDOs expressed in Rhodococcus strains that were difficult to obtain by the E. coli system showed different regiospecificities for dibenzofuran bioconversion as well as different substrate depletion specificities for chlorinated dioxins. Moreover, AhDO derived from R. erythropolis TA421 showed relatively diverse depletion-substrate specificity for chlorinated dioxins.
|
| 巻・号 |
73(4)
|
| ページ |
822-7
|
| 公開日 |
2009-4-23
|
| DOI |
10.1271/bbb.80680
|
| PII |
JST.JSTAGE/bbb/80680
|
| PMID |
19352040
|
| MeSH |
Actinomycetales / enzymology*
Bacterial Proteins / biosynthesis
Bacterial Proteins / genetics
Bacterial Proteins / metabolism
Benzofurans / isolation & purification
Benzofurans / metabolism*
Biodegradation, Environmental
Cloning, Molecular
Dioxins / isolation & purification
Dioxins / metabolism*
Dioxygenases / biosynthesis*
Dioxygenases / genetics
Dioxygenases / metabolism*
Environmental Pollutants / isolation & purification
Environmental Pollutants / metabolism*
Promoter Regions, Genetic
Rhodococcus / genetics*
Rhodococcus / metabolism
|
| IF |
1.516
|
| 引用数 |
5
|
|
WOS 分野
|
FOOD SCIENCE & TECHNOLOGY
CHEMISTRY, APPLIED
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 2892
JCM 3201 |
