論文 - 詳細
| RRC ID | 15741 |
|---|---|
| 著者 | Carvajal-Zarrabal O, Nolasco-Hipólito C, Bujang KB, Ishizaki A. |
| タイトル | Production of nisin Z using Lactococcus lactis IO-1 from hydrolyzed sago starch. |
| ジャーナル | J Ind Microbiol Biotechnol |
| Abstract |
A membrane bioreactor for production of nisin Z was constructed using Lactococcus lactis IO-1 in continuous culture using hydrolyzed sago starch as carbon source. A strategy used to enhance the productivity of nisin Z was to maintain the cells in a continuous growth at high cell concentration. This resulted in a volumetric productivity of nisin Z, as 50,000 IU l(-1) h(-1) using a cell concentration of 15 g l(-1), 30( degrees )C, pH 5.5 and a dilution rate of 1.24 h(-1). Adding 10 g l(-1) YE and 2 g l(-1) polypeptone, other inducers were unnecessary to maintain production of nisin. The operating conditions of the reactor removed nisin and lactate, thus minimizing their effects which allowed the maintenance of cells in continuous exponential growth phase mode with high metabolic activity. |
| 巻・号 | 36(3) |
| ページ | 409-15 |
| 公開日 | 2009-3-1 |
| DOI | 10.1007/s10295-008-0511-x |
| PMID | 19137340 |
| MeSH | Anti-Bacterial Agents / biosynthesis* Bioreactors Biotechnology / methods Culture Media Hydrolysis Lactococcus lactis / growth & development* Lactococcus lactis / metabolism Nisin / analogs & derivatives* Nisin / biosynthesis Starch / metabolism* |
| IF | 2.824 |
| 引用数 | 7 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| リソース情報 | |
| 一般微生物 | JCM 2504 JCM 7638 |