論文 - 詳細
| RRC ID | 15745 |
|---|---|
| 著者 | Miyajima Y, Satoh K, Umeda Y, Makimura K. |
| タイトル | Quantitation of fungal DNA contamination in commercial zymolyase and lyticase used in the preparation of fungi. |
| ジャーナル | Nihon Ishinkin Gakkai Zasshi |
| Abstract |
Small amounts of contaminants may lead to false-positive results in sensitive polymerase chain reaction (PCR) detection systems. To analyze contaminants and understand the usability of beta-glucanases in fungal preparations, we estimated the ribosomal DNA (rDNA) contamination in Zymolyase-100T and Lyticase by quantitative PCR. The amount of rDNA contamination determined by real-time PCR was 9210 copies/unit for Zymolyase-100T and 0.0323 copies/unit for Lyticase. The observations regarding these enzyme products indicate that careful consideration of contaminating DNA included in the reagents used for molecular diagnostics is necessary. |
| 巻・号 | 50(4) |
| ページ | 259-62 |
| 公開日 | 2009-1-1 |
| DOI | 10.3314/jjmm.50.259 |
| PII | JST.JSTAGE/jjmm/50.259 |
| PMID | 19942798 |
| MeSH | DNA, Fungal / analysis* DNA, Ribosomal / analysis* Drug Contamination* False Positive Reactions Fungi / genetics* Glucan Endo-1,3-beta-D-Glucosidase / chemistry* Glycoside Hydrolases Hydrolases / chemistry* Indicators and Reagents / chemistry* Multienzyme Complexes / chemistry* Peptide Hydrolases / chemistry* Polymerase Chain Reaction* |
| リソース情報 | |
| 一般微生物 | JCM 10253 |