Reference - Detail
| RRC ID | 15775 |
|---|---|
| Author | Tanioka Y, Miyamoto E, Yabuta Y, Ohnishi K, Fujita T, Yamaji R, Misono H, Shigeoka S, Nakano Y, Inui H, Watanabe F. |
| Title | Methyladeninylcobamide functions as the cofactor of methionine synthase in a Cyanobacterium, Spirulina platensis NIES-39. |
| Journal | FEBS Lett |
| Abstract |
To clarify the physiological function of pseudovitamin B(12) (or adeninylcobamide; AdeCba) in Spirulina platensis NIES-39, cobalamin-dependent methionine synthase (MS) was characterized. We cloned the full-length Spirulina MS. The clone contained an open reading frame encoding a protein of 1183 amino acids with a molecular mass of 132 kDa. Deduced amino acid sequences of the Spirulina MS contained critical residues identical to cobalamin-, zinc-, S-adenosylmethionine-, and homocysteine-binding motifs. The recombinant Spirulina enzyme showed higher affinity for methyladeninylcobamide than methylcobalamin as a cofactor. These results indicate that Spirulina cells can utilize AdeCba synthesized as the cofactor for MS. |
| Volume | 584(14) |
| Pages | 3223-6 |
| Published | 2010-7-16 |
| DOI | 10.1016/j.febslet.2010.06.013 |
| PII | S0014-5793(10)00503-X |
| PMID | 20558164 |
| MeSH | 5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase / chemistry 5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase / genetics 5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase / metabolism* Amino Acid Sequence Bacteria / genetics Bacteria / metabolism Cyanobacteria / genetics Cyanobacteria / metabolism* Inorganic Chemicals Molecular Sequence Data Organic Chemicals Protein Structure, Tertiary / genetics S-Adenosylmethionine / genetics S-Adenosylmethionine / metabolism Vitamin B 12 / analogs & derivatives Vitamin B 12 / genetics Vitamin B 12 / metabolism |
| IF | 3.057 |
| Times Cited | 20 |
| WOS Category | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| Resource | |
| General Microbes | JCM 6427 |
| Algae | NIES-39 |