| RRC ID |
15792
|
| 著者 |
Fukiya S, Sugiyama T, Kano Y, Yokota A.
|
| タイトル |
Characterization of an insertion sequence-like element, ISBlo15, identified in a size-increased cryptic plasmid pBK283 in Bifidobacterium longum BK28.
|
| ジャーナル |
J Biosci Bioeng
|
| Abstract |
The characteristics of mobile genetic elements in bifidobacteria are not well understood. We characterized an insertion sequence-like element of the IS200/IS605 family found in a size-increased cryptic plasmid in Bifidobacterium longum. During a plasmid profile analysis of B. longum BK strains, we encountered a 6.5-kbp cryptic plasmid pBK283 in B. longum BK28, the size of which has not been identified in bifidobacteria. Nucleotide sequence analysis indicated that an insertion sequence-like element was inserted into the 5.0-kbp pKJ50-like plasmid and resulted in a size increase of pBK283. The element, named ISBlo15, was 1593 bp in length and contained a single ORF encoding a putative transposase, which is similar to the transposase OrfB encoded by IS200/IS605 family elements. Several sequence characteristics, including conserved transposase motifs in OrfB and terminal palindromic sequences that differ from the typical terminal inverted repeats, strongly suggested that ISBlo15 is a member of the IS200/IS605 family. Sequences similar to ISBlo15 were widely distributed among the nine Bifidobacterium species tested, and those of highly homologous sequences were detected only in Bifidobacterium gallicum JCM8224(T).
|
| 巻・号 |
110(2)
|
| ページ |
141-6
|
| 公開日 |
2010-8-1
|
| DOI |
10.1016/j.jbiosc.2010.02.013
|
| PII |
S1389-1723(10)00076-9
|
| PMID |
20547379
|
| MeSH |
Base Sequence
Bifidobacterium / classification*
Bifidobacterium / genetics*
DNA Transposable Elements / genetics*
DNA, Bacterial / genetics*
Molecular Sequence Data
Plasmids / genetics*
Species Specificity
|
| IF |
2.366
|
| 引用数 |
8
|
|
WOS 分野
|
FOOD SCIENCE & TECHNOLOGY
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 1190
JCM 1192
JCM 1194
JCM 1200
JCM 1217
JCM 1222
JCM 1255
JCM 1275
JCM 8224 |
