論文 - 詳細
| RRC ID | 15792 |
|---|---|
| 著者 | Fukiya S, Sugiyama T, Kano Y, Yokota A. |
| タイトル | Characterization of an insertion sequence-like element, ISBlo15, identified in a size-increased cryptic plasmid pBK283 in Bifidobacterium longum BK28. |
| ジャーナル | J Biosci Bioeng |
| Abstract |
The characteristics of mobile genetic elements in bifidobacteria are not well understood. We characterized an insertion sequence-like element of the IS200/IS605 family found in a size-increased cryptic plasmid in Bifidobacterium longum. During a plasmid profile analysis of B. longum BK strains, we encountered a 6.5-kbp cryptic plasmid pBK283 in B. longum BK28, the size of which has not been identified in bifidobacteria. Nucleotide sequence analysis indicated that an insertion sequence-like element was inserted into the 5.0-kbp pKJ50-like plasmid and resulted in a size increase of pBK283. The element, named ISBlo15, was 1593 bp in length and contained a single ORF encoding a putative transposase, which is similar to the transposase OrfB encoded by IS200/IS605 family elements. Several sequence characteristics, including conserved transposase motifs in OrfB and terminal palindromic sequences that differ from the typical terminal inverted repeats, strongly suggested that ISBlo15 is a member of the IS200/IS605 family. Sequences similar to ISBlo15 were widely distributed among the nine Bifidobacterium species tested, and those of highly homologous sequences were detected only in Bifidobacterium gallicum JCM8224(T). |
| 巻・号 | 110(2) |
| ページ | 141-6 |
| 公開日 | 2010-8-1 |
| DOI | 10.1016/j.jbiosc.2010.02.013 |
| PII | S1389-1723(10)00076-9 |
| PMID | 20547379 |
| MeSH | Base Sequence Bifidobacterium / classification* Bifidobacterium / genetics* DNA Transposable Elements / genetics* DNA, Bacterial / genetics* Molecular Sequence Data Plasmids / genetics* Species Specificity |
| IF | 2.366 |
| 引用数 | 8 |
| WOS 分野 | FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 一般微生物 | JCM 1190 JCM 1192 JCM 1194 JCM 1200 JCM 1217 JCM 1222 JCM 1255 JCM 1275 JCM 8224 |