Reference - Detail
| RRC ID | 15807 |
|---|---|
| Author | Madhaiyan M, Poonguzhali S, Lee JS, Lee KC, Hari K. |
| Title | Bacillus rhizosphaerae sp. nov., an novel diazotrophic bacterium isolated from sugarcane rhizosphere soil. |
| Journal | Antonie Van Leeuwenhoek |
| Abstract |
A Gram-positive, non-pigmented, rod-shaped, diazotrophic bacterial strain, designated SC-N012(T), was isolated from rhizosphere soil of sugarcane and was subjected to a polyphasic taxonomic study. The strain exhibited phenotypic properties that included chemotaxonomic characteristics consistent with its classification in the genus Bacillus. Sequence analysis of the 16S rRNA gene of SC-N012(T) revealed the closest match (98.9% pair wise similarity) with Bacillus clausii DSM 8716(T). However, DNA-DNA hybridization experiments indicated low levels of genomic relatedness (32%) with this strain. The major components of the fatty acid profile are iso-C(15:0), anteiso-C(15:0), iso-C(17:0) and anteiso-C(17:0). The diagnostic cell-wall diamino acid was meso-diaminopimelic acid. The G+C content of the genomic DNA is 43.0 mol%. The lipids present in strain SC-N012(T) are diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol and two unknown phospholipids. Their predominant respiratory quinone was MK-7. Studies of DNA-DNA relatedness, morphological, physiological and chemotaxonomic analyses and phylogenetic data based on 16S rRNA gene sequencing allowed strain SC-N012(T) to be described as members of novel species of the genus Bacillus, for which the name Bacillus rhizosphaerae sp. nov. is proposed. The type strain is SC-N012(T) (=DSM 21911(T) = NCCB 100267(T)). |
| Volume | 100(3) |
| Pages | 437-44 |
| Published | 2011-10-1 |
| DOI | 10.1007/s10482-011-9600-3 |
| PMID | 21671194 |
| MeSH | Bacillus / classification* Bacillus / genetics Bacillus / isolation & purification* Bacillus / metabolism DNA, Bacterial / genetics DNA, Ribosomal / genetics Fatty Acids / metabolism Molecular Sequence Data Nitrogen Fixation* Phylogeny Rhizosphere* Saccharum / growth & development Saccharum / microbiology* Soil Microbiology* |
| IF | 2.674 |
| Times Cited | 16 |
| WOS Category | MICROBIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Wikipedia |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| General Microbes | JCM 13820 |