RRC ID 1581
Author Kumagai N, Ohno K, Tameshige R, Hoshijima M, Yogo K, Ishida N, Takeya T.
Title Induction of mouse c-src in RAW264 cells is dependent on AP-1 and NF-kappaB and important for progression to multinucleated cell formation.
Journal Biochem. Biophys. Res. Commun.
Abstract C-src is known to play an essential role in osteoclastogenesis. We studied the regulatory mechanism as well as the significance of c-src induction in RANKL-induced differentiation of mouse monocytic RAW264 cells to TRAP-positive-multinucleated cells. We determined the genomic organization of the 5'-terminal region of mouse c-src. Mutational and biochemical analyses in the region 0.9 kb upstream of the transcription start site revealed that c-Fos and JNK pathways, in addition to NF-kappaB, participate in c-src induction in response to RANKL. On the other hand, when the expression of c-src was suppressed by introducing antisense src, the number of multinucleated cells formed was significantly reduced. Together, these findings show that the expression of c-src is under the control of AP-1 and NF-kappaB in the differentiation of RAW264 cells and that c-src plays an essential role at the stage of progression to multinucleated cell formation.
Volume 325(3)
Pages 758-68
Published 2004-12-17
DOI 10.1016/j.bbrc.2004.10.094
PII S0006-291X(04)02433-7
PMID 15541355
MeSH Animals Carrier Proteins / pharmacology Cell Differentiation / drug effects Cell Differentiation / physiology Cell Proliferation / drug effects Dose-Response Relationship, Drug Gene Expression Regulation / drug effects Giant Cells / cytology* Giant Cells / metabolism* Macrophages / cytology* Macrophages / drug effects Macrophages / metabolism* Membrane Glycoproteins / pharmacology Mice NF-kappa B / metabolism* Proto-Oncogene Proteins pp60(c-src) / metabolism* RANK Ligand Receptor Activator of Nuclear Factor-kappa B Transcription Factor AP-1 / metabolism*
IF 2.559
Times Cited 13
DNA material CDMmMCSFCl7 (RDB01210)