論文 - 詳細
| RRC ID | 15812 |
|---|---|
| 著者 | Tanaka T, Kawabata H, Ogino C, Kondo A. |
| タイトル | Creation of a cellooligosaccharide-assimilating Escherichia coli strain by displaying active beta-glucosidase on the cell surface via a novel anchor protein. |
| ジャーナル | Appl Environ Microbiol |
| Abstract |
We demonstrated direct assimilation of cellooligosaccharide using Escherichia coli displaying beta-glucosidase (BGL). BGL from Thermobifida fusca YX (Tfu0937) was displayed on the E. coli cell surface using a novel anchor protein named Blc. This strain was grown successfully on 0.2% cellobiose, and the optical density at 600 nm (OD(600)) was 1.05 after 20 h. |
| 巻・号 | 77(17) |
| ページ | 6265-70 |
| 公開日 | 2011-9-1 |
| DOI | 10.1128/AEM.00459-11 |
| PII | AEM.00459-11 |
| PMID | 21742905 |
| PMC | PMC3165374 |
| MeSH | Actinomycetales / enzymology* Actinomycetales / genetics Biomass Cellobiose / metabolism Escherichia coli / enzymology* Escherichia coli / genetics Escherichia coli / growth & development Escherichia coli / metabolism* Membrane Proteins / genetics Membrane Proteins / metabolism Oligosaccharides / metabolism* Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Spectrophotometry beta-Glucosidase / genetics beta-Glucosidase / metabolism* |
| IF | 4.016 |
| 引用数 | 29 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY |
| リソース情報 | |
| 一般微生物 | JCM 20137 |