| RRC ID |
15815
|
| 著者 |
Yamamoto S, Sakai M, Inui M, Yukawa H.
|
| タイトル |
Diversity of metabolic shift in response to oxygen deprivation in Corynebacterium glutamicum and its close relatives.
|
| ジャーナル |
Appl Microbiol Biotechnol
|
| Abstract |
Oxygen-deprived Corynebacterium glutamicum R cells remain metabolically active, producing considerable amounts of organic acids even when not actively growing. We compared the proficiencies of C. glutamicum and close relatives grown under aerobic conditions to metabolize glucose when deprived of oxygen. Eight strains that readily consumed glucose without cell growth subsequently produced organic acids. Among these, the glucose consumption rates of the two C. glutamicum strains (>40 mM/h) and Corynebacterium efficiens (>12 mM/h) were an order of magnitude higher than those of the other five strains. The resultant organic acid yields of these three strains (>86%) consequently exceeded those of the other five (<60%). This difference is probably rooted in the comparatively inferior activities of glyceraldehyde-3-phosphate dehydrogenase, lactate dehydrogenase, and malate dehydrogenase observed in the five strains. Moreover, under oxygen deprivation, phosphoenolpyruvate carboxylase (PEPC) activity of C. efficiens was elevated tenfold, but its lack of fumarase activity meant that no succinic acid could be produced. The metabolic shift occasioned by addition of the PEPC substrate sodium bicarbonate resulted in a doubling of the glucose consumption rate of the two C. glutamicum strains but not that of the other six close relatives.
|
| 巻・号 |
90(3)
|
| ページ |
1051-61
|
| 公開日 |
2011-5-1
|
| DOI |
10.1007/s00253-011-3144-3
|
| PMID |
21327408
|
| MeSH |
Bacterial Proteins / genetics
Bacterial Proteins / metabolism
Corynebacterium / genetics
Corynebacterium / metabolism*
Corynebacterium glutamicum / genetics
Corynebacterium glutamicum / metabolism*
Glucose / metabolism
L-Lactate Dehydrogenase / genetics
L-Lactate Dehydrogenase / metabolism
Oxygen / metabolism*
|
| IF |
3.53
|
| 引用数 |
19
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 1305
JCM 12676
JCM 1332
JCM 15317
JCM 10987
JCM 12608
JCM 10112
JCM 13662
JCM 10676
JCM 13999 |
