| RRC ID |
1581
|
| 著者 |
Kumagai N, Ohno K, Tameshige R, Hoshijima M, Yogo K, Ishida N, Takeya T.
|
| タイトル |
Induction of mouse c-src in RAW264 cells is dependent on AP-1 and NF-kappaB and important for progression to multinucleated cell formation.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
C-src is known to play an essential role in osteoclastogenesis. We studied the regulatory mechanism as well as the significance of c-src induction in RANKL-induced differentiation of mouse monocytic RAW264 cells to TRAP-positive-multinucleated cells. We determined the genomic organization of the 5'-terminal region of mouse c-src. Mutational and biochemical analyses in the region 0.9 kb upstream of the transcription start site revealed that c-Fos and JNK pathways, in addition to NF-kappaB, participate in c-src induction in response to RANKL. On the other hand, when the expression of c-src was suppressed by introducing antisense src, the number of multinucleated cells formed was significantly reduced. Together, these findings show that the expression of c-src is under the control of AP-1 and NF-kappaB in the differentiation of RAW264 cells and that c-src plays an essential role at the stage of progression to multinucleated cell formation.
|
| 巻・号 |
325(3)
|
| ページ |
758-68
|
| 公開日 |
2004-12-17
|
| DOI |
10.1016/j.bbrc.2004.10.094
|
| PII |
S0006-291X(04)02433-7
|
| PMID |
15541355
|
| MeSH |
Animals
Carrier Proteins / pharmacology
Cell Differentiation / drug effects
Cell Differentiation / physiology
Cell Proliferation / drug effects
Dose-Response Relationship, Drug
Gene Expression Regulation / drug effects
Giant Cells / cytology*
Giant Cells / metabolism*
Macrophages / cytology*
Macrophages / drug effects
Macrophages / metabolism*
Membrane Glycoproteins / pharmacology
Mice
NF-kappa B / metabolism*
Proto-Oncogene Proteins pp60(c-src) / metabolism*
RANK Ligand
Receptor Activator of Nuclear Factor-kappa B
Transcription Factor AP-1 / metabolism*
|
| IF |
2.985
|
| 引用数 |
14
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 遺伝子材料 |
CDMmMCSFCl7 (RDB01210) |
