| RRC ID |
15844
|
| Author |
Zhang J, Wang J, Song F, Fang C, Xin Y, Zhang Y.
|
| Title |
Bacillus nanhaiisediminis sp. nov., an alkalitolerant member of Bacillus rRNA group 6.
|
| Journal |
Int J Syst Evol Microbiol
|
| Abstract |
A Gram-stain-positive, rod-shaped bacterium, designated strain NH3(T), was isolated from a sediment sample from the South China Sea and was subjected to a polyphasic taxonomic study. The isolate grew optimally at 37 °C and pH 9. Strain NH3(T) had cell-wall peptidoglycan based on meso-diaminopimelic acid and MK-7 as the predominant menaquinone. The cellular fatty acid profile included significant amounts of iso-C(15 : 0) and iso-C(14 : 0). The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The DNA G+C content of strain NH3(T) was 40.3 mol%. Phylogenetic analysis of the 16S rRNA gene sequence revealed that strain NH3(T) was a member of rRNA group 6 of the genus Bacillus, which includes alkalitolerant, alkaliphilic and halotolerant species. The closest phylogenetic relatives were Bacillus akibai 1139(T) (96.82 % 16S rRNA gene sequence similarity), B. pseudofirmus DSM 8715(T) (96.76 %), B. okhensis Kh10-101(T) (96.76 %) and B. alkalidiazotrophicus MS 6(T) (96.47 %). Strain NH3(T) could be distinguished from these phylogenetically close neighbours based on a number of phenotypic properties. On the basis of phenotypic and chemotaxonomic characteristics and phylogenetic data, we conclude that strain NH3(T) ( = CGMCC 1.10116(T) = JCM 16507(T)) merits classification as the type strain of a novel species, for which the name Bacillus nanhaiisediminis sp. nov. is proposed.
|
| Volume |
61(Pt 5)
|
| Pages |
1078-1083
|
| Published |
2011-5-1
|
| DOI |
10.1099/ijs.0.023671-0
|
| PMID |
20525818
|
| MeSH |
Alkalies / metabolism*
Bacillus / classification*
Bacillus / genetics
Bacillus / isolation & purification*
Bacillus / metabolism
Base Composition
DNA, Bacterial / genetics
DNA, Ribosomal / genetics*
Fatty Acids / metabolism
Geologic Sediments / microbiology*
Molecular Sequence Data
Phylogeny
RNA, Ribosomal, 16S / genetics
Seawater / microbiology*
|
| IF |
2.415
|
| Times Cited |
12
|
|
WOS Category
|
MICROBIOLOGY
|
| Resource |
| General Microbes |
JCM 16507
JCM 9157
JCM 13040 |
