RRC ID |
15882
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Author |
Cha IT, Oh YS, Park SJ, Park BJ, Lee JK, Lim CS, Park AR, Yoo JS, Lee DH, Rhee SK, Roh DH.
|
Title |
Reichenbachiella faecimaris sp. nov., isolated from a tidal flat, and emended descriptions of the genus Reichenbachiella and Reichenbachiella agariperforans.
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Journal |
Int J Syst Evol Microbiol
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Abstract |
A taxonomic study was carried out on two bacterial strains, PCP11(T) and PCP104, isolated from a tidal flat of the Yellow Sea, Korea. Comparative 16S rRNA gene sequence studies showed that these strains belonged to the family Cytophagaceae, phylum Bacteroidetes. Strains PCP11(T) and PCP104 shared 99.4 % sequence similarity and were related most closely to Reichenbachiella agariperforans KMM 3525(T) (95.8 and 96.0 % sequence similarity, respectively). Members of the genera Fulvivirga, Roseivirga, Fabibacter and Marinoscillum were the next closest relatives of the new isolates, with sequence similarities ≤ 91 %. The two isolates were Gram-staining-negative, strictly aerobic, gliding bacteria. They grew in the presence of 1-5 % NaCl, at pH 5.5-8.5 and at 4-35 °C. Strains PCP11(T) and PCP104 shared a number of physiological and biochemical properties with Reichenbachiella agariperforans KMM 3525(T), but they differed from this strain in the hydrolysis of biopolymers and in the production of carotenoid and flexirubin-type pigments. Both strains possessed iso-C(15 : 0), summed feature 4 (C(16 : 1)ω7c and/or iso-C(15 : 0) 2-OH) and C(15 : 0) as major cellular fatty acids. The major respiratory quinone was menaquinone 7 (MK-7). The G+C contents of the genomic DNA of strains PCP11(T) and PCP104 were 39.6 and 41.9 mol%, respectively. On the basis of phenotypic data and phylogenetic inference, it is proposed that the two isolates represent a novel species, Reichenbachiella faecimaris sp. nov., with strain PCP11(T) ( = KACC 14523(T) = JCM 16588(T)) as the type strain. Emended descriptions of the genus Reichenbachiella and Reichenbachiella agariperforans are also proposed.
|
Volume |
61(Pt 8)
|
Pages |
1994-1999
|
Published |
2011-8-1
|
DOI |
10.1099/ijs.0.026849-0
|
PMID |
20851915
|
MeSH |
Cytophagaceae / classification*
Cytophagaceae / genetics
Cytophagaceae / isolation & purification*
Cytophagaceae / metabolism
DNA, Bacterial / genetics
DNA, Ribosomal / genetics
Fatty Acids / metabolism
Molecular Sequence Data
Phylogeny
RNA, Ribosomal, 16S / genetics
Seawater / microbiology*
|
IF |
2.415
|
Times Cited |
8
|
WOS Category
|
MICROBIOLOGY
|
Resource |
General Microbes |
JCM 16588 |