論文 - 詳細
| RRC ID | 1590 |
|---|---|
| 著者 | Ogawa F, Iinuma H, Okinaga K. |
| タイトル | Dendritic cell vaccine therapy by immunization with fusion cells of interleukin-2 gene-transduced, spleen-derived dendritic cells and tumour cells. |
| ジャーナル | Scand J Immunol |
| Abstract |
We examined the preventive and therapeutic effects of fusion cells prepared from spleen-derived dendritic cells (DCs) transduced with the interleukin-2 (IL-2) gene and QRsP fibrosarcoma cells in a mouse lung metastasis model. The IL-2 or LacZ gene was introduced into spleen-derived DCs using an adenoviral vector. Irradiated QRsP tumour cells were fused with IL-2 gene-transduced DCs (fusion/IL-2) or LacZ gene-transduced DCs (fusion/LacZ) by polyethylene glycol. These fusion cells expressed major histocompatibility complexes (MHC) class I and II, CD86, CD11c and CD8alpha. Splenocytes from mice vaccinated with fusion cells showed increased production of interferon-gamma (IFN-gamma) and cytotoxic T-lymphocyte (CTL) activity as compared with those vaccinated with DCs or tumour cells alone, and CTL levels were higher in fusion/IL-2-vaccinated mice than in fusion/LacZ-vaccinated mice. In our experiments on the protective and therapeutic effects on lung metastasis, mice vaccinated with fusion/IL-2 fusion/LacZ or fusion showed a significant reduction in pulmonary metastasis compared with those given DCs, tumour or phosphate-buffered saline. The introduction of the IL-2 gene into fusion cells produced more potent preventive and therapeutic effects. These results suggest that immunization with fusion cells prepared from spleen-derived DCs and tumour cells is capable of inducing preventive and therapeutic anti-tumour immunity against lung metastasis, and modification by the IL-2 gene may increase anti-tumour efficacy. |
| 巻・号 | 59(5) |
| ページ | 432-9 |
| 公開日 | 2004-5-1 |
| DOI | 10.1111/j.0300-9475.2004.01411.x |
| PII | SJI1411 |
| PMID | 15140052 |
| MeSH | Animals Cancer Vaccines / immunology* Cell Fusion Cell Line, Tumor Cytotoxicity Tests, Immunologic Dendritic Cells / immunology* Fibrosarcoma / genetics Fibrosarcoma / immunology Fibrosarcoma / therapy* Flow Cytometry Immunotherapy, Active* Interferon-gamma / biosynthesis Interleukin-2 / genetics* Interleukin-4 / biosynthesis Lung Neoplasms / prevention & control* Lung Neoplasms / secondary Lymphocytes / immunology Male Mice Neoplasm Metastasis / prevention & control Spleen / cytology Transduction, Genetic |
| IF | 2.717 |
| 引用数 | 19 |
| WOS 分野 | IMMUNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 遺伝子材料 | AxCAmIL2 (RDB1402) |