| RRC ID |
15912
|
| 著者 |
Morita H, Nakano A, Onoda H, Toh H, Oshima K, Takami H, Murakami M, Fukuda S, Takizawa T, Kuwahara T, Ohno H, Tanabe S, Hattori M.
|
| タイトル |
Bifidobacterium kashiwanohense sp. nov., isolated from healthy infant faeces.
|
| ジャーナル |
Int J Syst Evol Microbiol
|
| Abstract |
Strains HM2-1 and HM2-2(T) were isolated from the faeces of a healthy infant and were characterized by determining their phenotypic and biochemical features and phylogenetic positions based on partial 16S rRNA gene sequence analysis. They were Gram-positive, obligately anaerobic, non-spore-forming, non-gas-producing, and catalase-negative non-motile rods. They did not grow at 15 or 45 °C in anaerobic bacterial culture medium, and their DNA G+C content was in the range 56-59 mol%. In enzyme activity tests, strains HM2-1 and HM2-2(T) were positive for α/β-galactosidases and α/β-glucosidases but negative for β-glucuronidase and cystine arylamidase. An analysis of the cell-wall composition of strains HM2-1 and HM2-2(T) revealed the presence of glutamic acid, alanine and lysine. The presence of fructose-6-phosphate phosphoketolase shows that isolates HM2-1 and HM2-2(T) are members of the genus Bifidobacterium. These two isolates belong to the same species of the genus Bifidobacterium. Strain HM2-2(T) was found to be related to Bifidobacterium catenulatum JCM 1194(T) (97.4 % 16S rRNA gene sequence identity: 1480/1520 bp), Bifidobacterium pseudocatenulatum JCM 1200(T) (97.2 %: 1472/1514 bp), Bifidobacterium dentium ATCC 27534(T) (96.7 %: 1459/1509 bp) and Bifidobacterium angulatum ATCC 27535(T) (96.5 %: 1462/1515 bp). The predominant cellular fatty acids of strains HM2-1 and HM2-2(T) were 16 : 0 and 18 : 1ω9c, with proportions greater than 18 % of the total. Phylogenetic analyses involving phenotypic characterization, DNA-DNA hybridization and partial 16S rRNA gene sequencing proves that the strains represent a novel species of the genus Bifidobacterium, for which the name Bifidobacterium kashiwanohense sp. nov. is proposed. The type strain is HM2-2(T) ( = JCM 15439(T) = DSM 21854(T)).
|
| 巻・号 |
61(Pt 11)
|
| ページ |
2610-2615
|
| 公開日 |
2011-11-1
|
| DOI |
10.1099/ijs.0.024521-0
|
| PMID |
21148680
|
| MeSH |
Bacterial Proteins / genetics
Bacterial Proteins / metabolism
Base Composition
Bifidobacterium / classification*
Bifidobacterium / genetics
Bifidobacterium / isolation & purification*
Cellulases / genetics
Cellulases / metabolism
DNA, Bacterial / genetics
Fatty Acids / metabolism
Feces / microbiology*
Humans
Infant
Male
Molecular Sequence Data
Phylogeny
RNA, Ribosomal, 16S / genetics
beta-Galactosidase / genetics
beta-Galactosidase / metabolism
|
| IF |
2.415
|
| 引用数 |
21
|
|
WOS 分野
|
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 1194
JCM 1195
JCM 1200
JCM 7096
JCM 15439 |
